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Analysis of Protein Interactions and Mutations in Yeast Using Co-IP Techniques

This study investigates the interactions among various proteins in *Saccharomyces cerevisiae* through co-immunoprecipitation (Co-IP) methods. We focus on key proteins like Sir2, Hst1, Hst2, and Sum1, along with their mutant variants. The analysis includes relative protein expression levels and interaction dynamics under different genetic backgrounds (wild-type and specific gene deletions). By exploring the protein network, we aim to enhance the understanding of regulatory mechanisms in yeast cells.

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Analysis of Protein Interactions and Mutations in Yeast Using Co-IP Techniques

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  1. (a) tQ(UUG)H tE(UUC)E1 SNR30 500 bp 500 bp 500 bp [0- 1093] Input [0- 666] Sir2 [0- 500] Hst1 [0- 565] Sum1 [0- 84] Hst2 tQ(UUG)H YHR015W tE(UUC)E1 YER012W SNR30 YLR028C (b) tQ(UUG)H SNR30 Relative IP Relative IP Sir2-myc Sir2-myc untagged untagged Hst2-myc Hst1-myc Hst2-myc Hst1-myc Sum1-myc Sum1-myc (c) tE(UUC)E1 tQ(UUG)H untagged untagged WT WT sir2∆ sir2∆ hst1∆ hst1∆ sum1∆ sum1∆ Relative IP Relative IP Mcd1-myc Smc4-myc Brn1-myc Mcd1-myc Smc4-myc Brn1-myc

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