Sir2 - PowerPoint PPT Presentation

Sirulins

Sirulins. A&amp;S300-002 Jim Lund. Ageless Quest: One Scientist's Search for Genes That Prolong Youth. Leonard Guarente. Functions of yeast SIR2. The structure of the nucleolus. For silencing near chromosome telomeres. Distinct from repression.

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hst1∆

(a). Tel_ChrXV_L. 100 bp. Input. [0- 8210]. Sir2. [0- 5000]. Hst1. [0- 3741]. Sum1. [0- 4242]. Hst2. [0- 635]. Hst3. [0- 4594]. Hst4. [0- 1796]. TG 1-3 118 bp. ( b ). Tel_ChrVIII_R. 100 bp. Input. [0- 8210]. Sir2. [0- 5000]. Hst1. [0- 3741]. Sum1. [0- 4242]. Hst2.

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(a)

(a). ( b ). PDC1. 3.5. WT. 3. npt1∆. 2.5. 2. NAD/NADH ratio. Relative expression. 1.5. 1. 0.5. 0. log. 3. 6. 9. 12. log. 3. 6. 9. 12. hrs post log phase. hrs post log phase. ( c ). ( d ). [0- 1093]. 500 bp. Input. WT. [0- 666]. sir2∆. hst1∆. Sir2. x. sum1∆.

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Sirtuin Signaling Pathway

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