Chapter 13 Lecture 1. Enzymes--Kinetics and Specificity. Three key enzyme characteristics. Catalytic Power Rate enhancements as much as 10 15 to 10 17 Kinetic control over thermodynamic potentiality Specificity
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Enzymes--Kinetics and Specificity
They show coenzymes and which types of molecules they act upon.
Finally, there is a list of a few enzymes that use coenzymes.
Table 13-2, p.409
What can kinetics tell us?
Note that the instantaneous slope, the reaction rate or velocity, decreases with time, because there is less reactant there as the reaction progresses.
This is an exponential decay--note that 1/2 of A is remaining at the first half life, t1/2.
Fig. 13-04, p.410
Active learning: Which reaction would you expect to go with a faster rate?
Fig. 13-01, p.406
Fig. 13-05a, p.411
Active learning: For the unimolecular reaction AP, what
would you expect to see if you plotted velocity versus the
[A]? (Note for enzyme-catalyzed it’s very different)
Fig. 13-06, p.412
And [ES] can be ignored
either k-2 = 0, or [P] = 0
(The binding of S to E is rapid compared to the breakdown of the ES complex.)
This equation is hyperbolic.
change during the course of the
Active learning: Can you explain what happens and why to S, P, E and ES as a function of time?
This lower plot in part b is an enlargement of the beginning of the curves from part a.
Fig. 13-08, p.413
Based on mechanism: