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This study uses EcoRV, AflII, BsrGI, NdeI restriction enzymes to analyze a 9.22Kb region on Chr4. The 2.4Kb PGK-Neo gene is inserted at specified points. Probes help locate targeted DNA sequences.
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SupFig 1 A STOP AflII AflII EcoRV 9.22Kb+1.9Kb+71bp Probe1 BsrGI BsrGI E19a NdeI NdeI B PGK-Neo 2.4Kb 3.8Kb Chr4 FRT FRT LoxP LoxP 1.9Kb 1Kb 151 157 700 101 300 71 66 140 F1 R1 F2 R2