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CH 3

HO. OH. B. CH 3. CH 3. HO. HO. O. O. HO. OH. OH. CH 3. HO. HO. O. O. O. CH 3. HO. O. HO. HO. O. HO. R -THMF. MHF. S -THMF. OH. CH 3. O. O. -. CH 3. OH. O. O. HO. HO. R -DHMF. S -DHMF. OH. O. Pfs. OH. O. LuxS. DPD. LuxP Ligand. LsrB Ligand. Toxic

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CH 3

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  1. HO OH B CH3 CH3 HO HO O O HO OH OH CH3 HO HO O O O CH3 HO O HO HO O HO R-THMF MHF S-THMF OH CH3 O O - CH3 OH O O HO HO R-DHMF S-DHMF OH O Pfs OH O LuxS DPD LuxP Ligand LsrB Ligand Toxic Metabolite AI-2 is a group of molecules -2 H2O + B(OH)4- S-THMF-borate -H2O -H2O H2O H2O Fig 2 AI-2 is made by LuxS SAH X-CH3 SRH X LuxS has metabolic function OA HCY SAM MetB CysK MET SO42- S2- CYS protein Activated Methyl Cycle

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