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3000 2500 2000 1500 1000 500 0 -500

3000 2500 2000 1500 1000 500 0 -500. A. PCR baseline subtracted CF RFU. 10 pg. 100 fg. 1 pg. 1 fg. 10 fg. 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42. Cycle number. 34 30 26 22 18.

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3000 2500 2000 1500 1000 500 0 -500

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  1. 3000 2500 2000 1500 1000 500 0 -500 A PCR baseline subtracted CF RFU 10 pg 100 fg 1 pg 1 fg 10 fg 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 Cycle number 34 30 26 22 18 Co-relation Coefficient 1.0, Slope: -3.423, Intercept: 33.503 Y=3.423X + 33.503 PCR efficiency 96.0%, B Threshold cycle (Ct) 1 2 3 4 5 6 Starting Copy Number (Log) 4x108 3x108 2x108 1x108 1x104 I II C JCV T-antigen gene copy number 0 10 20 30 40 50 60 70 HA units

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