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Microscopes

Microscopes. What is this?. I. Parts of the Compound Light Microscope. Metal or plastic part on which microscope rests BASE. Somewhat C-shaped pillar arising from the base and supports the stage and ocular components. Used for carrying. ARM.

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Microscopes

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  1. Microscopes

  2. What is this?

  3. I. Parts of the Compound Light Microscope Metal or plastic part on which microscope rests BASE

  4. Somewhat C-shaped pillar arising from the base and supports the stage and ocular components. Used for carrying ARM

  5. Flat platform attached to lower portion of arm on which slides or samples are STAGE

  6. Collects light rays and focuses them on the specimen slide. Can be adjusted for better resolution (sharper image) DIAPHRAGM

  7. Convex lens used to magnify the image; generally 10-15x magnification Ocular or eyepiece

  8. Small convex lenses screwed into nosepiece and increase the magnification of the sample. Low power = 4x high power =40x OBJECTIVES

  9. Larger knob which moves the base rapidly up or down to get the sample into coarse focus COARSE ADJUSTMENT KNOB

  10. Smaller knob which moves the base slowly through short distances to get the sample into sharp focus FINE ADJUSTMENT KNOB

  11. REMEMBER: • All specimens must be thin for LIGHT to shine through them so that you can see clearly.

  12. REMEMBER • Only use the FINE ADJUSTMENT KNOB when focusing on medium or high power objectives!! • This avoids damaging or breaking the slide

  13. Electron microscope • Electron microscope – a microscope that focuses beams of electrons instead of light rays. Produces clearer, 3D images.

  14. Muscle Tissue!

  15. LIGHT MICROSCOPE VS. ELECTRON MICROSCOPE

  16. Calculating magnification • Just multiply the objective lens power by the ocular lens power • Lower power: 10 X 4= 40 • Medium power: 10 x10= 100 • High power: 10x40=400

  17. Guided Practice • Instructions: You will be using your fingers to show me the correct answer for the following questions We are going to read the question SILENTLY. DO NOT CALL OUT THE ANSWER. Think about the question and pick an answer. When I count to 3, You are going to hold up your choice. DO NOT LOOK AT ANYONE ELSE’S ANSWER!! THIS IS YOUR TIME TO FIGURE OUT IF YOU KNOW THE INFORMATION!

  18. QUESTION 1 1. One advantage of using a light microscope is that it – A. has high resolution B. magnifies clearly to 1000x C. can be used to view living cells D. can be used to view atoms

  19. QUESTION 1 1. One advantage of using a light microscope is that it – A. has high resolution B. magnifies clearly to 1000x C. can be used to view living cells D. can be used to view atoms

  20. QUESTION 2 2. When you are focusing on medium or high power objectives, you should use the • Coarse knob adjustment • Fine knob adjustment • Ocular • Diaphargm

  21. QUESTION 2 2. When you are focusing on medium or high power objectives, you should use the • Coarse knob adjustment • Fine knob adjustment • Ocular • Diaphargm

  22. Question 3 The purpose of the diaphragm is to • Adjust the amount of light focusing on the specimen • Magnify the specimen on the stage • Adjust the magnification of the specimen • View the specimen on the stage

  23. Question 3 The purpose of the diaphragm is to • Adjust the amount of light focusing on the specimen • Magnify the specimen on the stage • Adjust the magnification of the specimen • View the specimen on the stage

  24. Question 4 If a microscope’s ocular is marked 10x and the low power objective is marked 4x, what would be the total magnification when using the low power objective? A. 10x C. 40x B. 12x D. 400x

  25. Question 4 If a microscope’s ocular is marked 10x and the low power objective is marked 4x, what would be the total magnification when using the low power objective? A. 10x C. 40x B. 12x D. 400x

  26. Question 5 If a microscope’s ocular is marked 10x and the low power objective is marked 30x, what would be the total magnification when using the low power objective? • 3x • 300x • 30x • 3000x

  27. Question 5 If a microscope’s ocular is marked 10x and the low power objective is marked 30x, what would be the total magnification when using the low power objective? • 3x • 300x • 30x • 3000x

  28. Question 6 One disadvantage of using an electron microscope is that it A. has high resolution B. Very expensive C. shows details of the surface D. can be used to view atoms

  29. Question 6 One disadvantage of using an electron microscope is that it A. has high resolution B. Very expensive C. shows details of the surface D. can be used to view atoms

  30. Worktime • How should you carry a microscope? • What knob should you use to focus when using a high power objective? • Why should you not use the coarse knob adjustment when using the high power objective?

  31. Worktime • How should you carry a microscope? • What knob should you use to focus when using a high power objective? • Why should you not use the coarse knob adjustment when using the high power objective? • What is the purpose of the diaphragm? • Where is the ocular lens located? • Where are the objective lens located • What is the purpose of the stage? • If the ocular lens has a magnification of 10x and the objective lens has a magnification of 25x, what is the total magnification? • If the ocular lens has a magnification of 4x and the objective lens has a magnification of 50x, what is the total magnification? • If the ocular lens has a magnification of 5x and the objective lens has a magnification of 100x, what is the total magnification?

  32. Worktime • How should you carry a microscope? • What knob should you use to focus when using a high power objective? • Why should you not use the coarse knob adjustment when using the high power objective? • What is the purpose of the diaphragm? • Where is the ocular lens located? • Where are the objective lens located • What is the purpose of the stage? • If the ocular lens has a magnification of 10x and the objective lens has a magnification of 25x, what is the total magnification? • If the ocular lens has a magnification of 4x and the objective lens has a magnification of 50x, what is the total magnification? • If the ocular lens has a magnification of 5x and the objective lens has a magnification of 100x, what is the total magnification?

  33. Exit Ticket:1 • I used to work at an Air force base. We would work with particles of metal called nano particles. The particles were dead, and microscopic. What would be the best way to see those? • A light microscope • An electron scanning microscope • A light telescope • An electron scanning teloscope

  34. Exit Ticket 2: 2) When the low power objective is being used you should adjust it using the: a) fine adjustment knob b) diaphragm c) course adjustment knob d) ocular adjustment knob

  35. Exit Ticket 3 3) Which of the following statements is NOT true about objects viewed with an electron scanning microscope? A. They must be thin enough to allow light to pass through them. B. They must be living specimens C. No dyes or states can be used on the objects D. They must be made of organic material

  36. Exit Ticket 4 4) If the microscopes ocular lens is marked 10x and the lower power objective is marked 5x, what is the total magnification? a) 10 b) 5 c) 50 d) 500

  37. Exit Ticket: 5 5) What is the proper way to place a cover slip on a wet mount slide? a) c) b) d)

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