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CI-710 Miniature Spectrometer. Tuesday, October 22, 2013 Conducted by: Brienne Meyer bmeyer@cid-inc.com. Agenda/Outline. CI-710 hardware CI-710 Theory of Operation SpectraSnap! Software Competing Instruments. Selling Points. Three points to make Accuracy Portability Ease of Use.

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ci 710 miniature spectrometer

CI-710 Miniature Spectrometer

Tuesday, October 22, 2013

Conducted by: Brienne Meyer

bmeyer@cid-inc.com

agenda outline
Agenda/Outline
  • CI-710 hardware
  • CI-710 Theory of Operation
  • SpectraSnap! Software
  • Competing Instruments
selling points
Selling Points

Three points to make

  • Accuracy
  • Portability
  • Ease of Use
ci 710 miniature leaf spectrometer
CI-710 Miniature Leaf Spectrometer
  • Truly portable
  • Field-ready with leaf probe
  • Wide-range spectrum
    • 400nm-950nm (PAR & NIR)
  • High-resolution (~1.5nm)
  • Real-time display
  • Lightweight (~290g)
what does the ci 710 measure
What does the CI-710 measure?
  • Transmission/absorbance mode:
    • Light passing through the leaf
  • Reflectance mode:
    • light reflecting from leaf surface

Source: www.ceramicx.com/infrared-heat/

ci 710 theory of operation
CI-710 Theory of Operation
  • Bifurcating fiber optic cable
    • Range 400-950nm
  • Reference light beam from 2 sources
    • Blue LED & Incandescent lamp
  • Output in the visible-to- infrared range
ci 710 theory continued
CI-710 Theory Continued
  • Most photosynthetically active green leaves:
    • strongly absorb blue/blue-green and red light
    • reflect green and yellow light

Source: scienceprojectideasforkids.com

Source: www.shortcourses.com

ci 710 theory iii
CI-710 Theory III
  • Light source has 2 positions:
    • transmission/absorbance or reflectance mode

Transmission and

Absorbance Mode

Reflectance Mode

  • Light travels through fiber optic to leaf probe and leaf
ci 710 theory iv
CI-710 Theory IV
  • Disperses light by a diffraction grating
  • Projects the dispersed light onto a CCD array
  • Each pixel corresponds to a specific wavelength of light
  • SpectraSnap! displays light intensity of each pixel

Source: www.horiba.com

ci 710 leaf clip
CI-710 Leaf Clip
  • Also referred to as leaf probe
  • Isolates stray light
  • Secures specimen
  • In transmission or absorbance mode,
  • the leaf clip scatters
  • the source beam
ci 710 applications
CI-710 Applications
  • Measures light transmission, absorption and reflection of leaves
  • Measure optical properties of glass
  • WengJH. et al. RELATIONSHIPS BETWEEN PHOTOSYSTEM II EFFICIENCY AND PHOTOCHEMICAL REFLECTANCE INDEX UNDER DIFFERENT LEVELS OF ILLUMINATION: COMPARISON AMONG SPECIES GROWN AT HIGH-AND LOW ELEVATIONS THROUGH DIFFERENT SEASONS. Trees-Structure and Function, volume 26, issue 2, pages 343-351, April 2012
  • National Chung Hsing University, Taiwan
ci 710 applications continued
CI-710 Applications Continued
  • Qualitative spectral data from interaction of light with leaf
  • Pigments, organic compounds, and inorganic chemicals exhibit distinct wavelength characteristics

Common Plant Pigments

  • Leaf surface characterization
  • Photochemical reactions
  • Index of refraction

Source: www.cameratechnica.com

ci 710 general points
CI-710 General Points
  • USB powered by tablet or laptop PC:
    • SpectraSnap!
      • Waveform and wavelength values
      • Peaks
      • Pre-loaded

indices

SpectraSnap!

spectrasnap
SpectraSnap!
  • Driver installation built-into software
  • Analyze multiple peaks
  • Integrate across wavelength bands
  • Compare specimens
    • Add layers
    • Import previously

saved layers

spectrasnap indices
SpectraSnap! Indices
  • Normalized Difference Vegetation Index (NDVI)
  • Plant Senescence Reflectance Index (PRSI)
  • Pigments: Anthocyanin, Carotenoids
  • Add custom algorithms/indices
  • Simultaneously monitor and record indices

Source: www.exelisvis.com

ci 710 tips
CI-710 Tips
  • Warm-up time: 15-30 minutes
  • Recalibrate often
  • Use reflectance standard to calibrate in reflectance mode
    • white circle faces down
  • Use a black backing on other side of leaf in reflectance mode
how to demonstrate the ci 710
How to Demonstrate the CI-710
  • Warm up CI-710
    • Connect to PC, light turned on
  • Calibrate
  • Open leaf clip
  • Position leaf
  • View data in SpectraSnap!
  • Add calculations
  • Export to spreadsheet
competing instruments
Competing Instruments

Source: www.stellarnet.us

Source: www.asdi.com

conclusion
Conclusion
  • Fast, reliable, non-destructive measurements
  • How to demonstrate a leaf spectroscopy measurement
    • SpectraSnap! software
    • Free & easy to use
  • Wide spectrum (400-950nm)