Basic Genetic Stuff. A. Chromosomes- Cytogenetics. Metaphase Spread. Metaphase was first observed in 1956. Chromosomes are visible in this stage of cell division. The chromosome number in all humans is 46. The metaphase spread procedure is used in DNA analysis. Metaphase Spread.
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A. Chromosomes- Cytogenetics
In three easy steps:
2: Binding pattern
3: Centromere position