PCR Electrophoresis. By C. Kohn, Agricultural Sciences, Waterford WI. Genetic Testing. The Sanger Method enables scientists to read DNA letter by letter. This test made the Human Genome Project, and the sequencing of many other species, possible.
By C. Kohn, Agricultural Sciences, Waterford WI
PCR works first by separating the dual-strand of DNA into two individual strands using heat.
PCR Animations 1, 2, 3, 4, 5
After amplification by Taq polymerase, each DNA sample is cut up into fragments by restriction enzymes (chemical scissors)
The same restriction enzyme will cut the same gene from different people in different ways.Chunks of DNA will be larger if they have longer STR repeats.