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PCR - PowerPoint PPT Presentation


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PCR. PCR AMPLIFICATION. PCR APPLICATIONS. MOLECULAR BIOLOGY ENVIRONMENTAL SCIENCE FORENSIC/MEDICAL SCIENCE BIOTECHNOLOGY GENETICS GENE CLONING MICROBIOLOGY. DNA STRUCTURE. SUGAR-PHOSPHATE BACKBONE A=ADENINE T=THYMINE C=CYTOSINE G=GUANINE. PCR REQUIREMENTS. DNA PRIMERS

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PCR


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    Presentation Transcript
    1. PCR

    2. PCR AMPLIFICATION

    3. PCR APPLICATIONS • MOLECULAR BIOLOGY • ENVIRONMENTAL SCIENCE • FORENSIC/MEDICAL SCIENCE • BIOTECHNOLOGY • GENETICS • GENE CLONING • MICROBIOLOGY

    4. DNA STRUCTURE • SUGAR-PHOSPHATE BACKBONE • A=ADENINE • T=THYMINE • C=CYTOSINE • G=GUANINE

    5. PCR REQUIREMENTS • DNA • PRIMERS • dATP, dTTP, dCTP, dGTP • POLYMERASES

    6. RAPD PCR • RANDOM • AMPLIFIED • POLYMORPHIC • DNA

    7. RAPD APPLICATIONS • DNA FINGERPRINTING • ANALYSIS OF ANCIENT DNA FROM FOSSILS • MAPPING THE HUMAN GENOME • FINGERPRINTING UNKNOWN MICROORGANISMS

    8. RAPD PRIMERS • 10 NUCLEOTIDES LONG • ARBITRARY SEQUENCE • BINDS AT MULTIPLE SEQUENCES ALONG DNA • NON-SPECIFIC BINDING • ACCURACY REQUIRES AT LEAST 10 PRIMERS