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PCR

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PCR - PowerPoint PPT Presentation


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PCR. PCR AMPLIFICATION. PCR APPLICATIONS. MOLECULAR BIOLOGY ENVIRONMENTAL SCIENCE FORENSIC/MEDICAL SCIENCE BIOTECHNOLOGY GENETICS GENE CLONING MICROBIOLOGY. DNA STRUCTURE. SUGAR-PHOSPHATE BACKBONE A=ADENINE T=THYMINE C=CYTOSINE G=GUANINE. PCR REQUIREMENTS. DNA PRIMERS

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Presentation Transcript
pcr applications
PCR APPLICATIONS
  • MOLECULAR BIOLOGY
  • ENVIRONMENTAL SCIENCE
  • FORENSIC/MEDICAL SCIENCE
  • BIOTECHNOLOGY
  • GENETICS
  • GENE CLONING
  • MICROBIOLOGY
dna structure
DNA STRUCTURE
  • SUGAR-PHOSPHATE BACKBONE
  • A=ADENINE
  • T=THYMINE
  • C=CYTOSINE
  • G=GUANINE
pcr requirements
PCR REQUIREMENTS
  • DNA
  • PRIMERS
  • dATP, dTTP, dCTP, dGTP
  • POLYMERASES
rapd pcr
RAPD PCR
  • RANDOM
  • AMPLIFIED
  • POLYMORPHIC
  • DNA
rapd applications
RAPD APPLICATIONS
  • DNA FINGERPRINTING
  • ANALYSIS OF ANCIENT DNA FROM FOSSILS
  • MAPPING THE HUMAN GENOME
  • FINGERPRINTING UNKNOWN MICROORGANISMS
rapd primers
RAPD PRIMERS
  • 10 NUCLEOTIDES LONG
  • ARBITRARY SEQUENCE
  • BINDS AT MULTIPLE SEQUENCES ALONG DNA
  • NON-SPECIFIC BINDING
  • ACCURACY REQUIRES AT LEAST 10 PRIMERS
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