Comparative MS/MS Analysis of GSNO Standard and ARPE-19 Lysate Exposed to Normal Glucose Conditions

Jul 01, 2025

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This supplementary figure displays the MS/MS spectra of GSNO standard and ARPE-19 cell lysate under normal glucose conditions. Explore the molecular analysis of compounds in the samples.

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Comparative MS/MS Analysis of GSNO Standard and ARPE-19 Lysate Exposed to Normal Glucose Conditions

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Supplementary Figure 3: (A) MS/MS of GSNO standard. (B) MS/MS of total lysate of ARPE-19 cells exposed to normal glucose conditions.

Supplementary Figure 3

Supplementary Figure 3. A. B. Dry weight of grain in a panicle (g/panicle) .

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Supplementary Figure 1

Supplementary Figure 1 Examples of fluorescence images of SFs (A, D), nesprin 1 (B, E), and their merged images (C, F) on the apical and basal sides of SMCs , respectively, to confirm the distribution of nesprin 1, Bar = 10 µm. J. K. P < 0.05. P < 0.05. Frequency of cells

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Supplementary Figure 1

**. Hs578T Cells. Supplementary Figure 1.

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Supplementary Figure S3

Supplementary Figure S3. A. B. HCT116 Cells. SW480 Cells. Cell Size (µm). Cell Size (µm). HMGA1 Knock-down. Control. Control. HMGA1 Knock-down. 100 µm. 100 µm. 100 µm. 100 µm.

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Supplementary Figure 2

Supplementary Figure 2. Supplementary Figure 2 . qRT -PCR-based analysis of LPA and LPC receptor expression in ARMs and ERMS cells lines. Panel A - LPAR1, LPAR2, and LPAR3 receptors. Panel B - G2A and GPR4 receptors. Combined data from two independent experiments are shown. A. B.

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Supplementary figure S1

A. Supplementary figure S1. B. C. D. Figure S1. EGF in combination with LY294002 induces survival and NE differentiation of LNCaP cells.

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Supplementary Figure 1

Article title: PI3K independent activation of mTORC1 as a target in lapatinib-resistant ERBB2+ breast cancer cells Journal: Breast Cancer Research and Treatment

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Supplementary Figure 3

Supplementary Figure 3. Telomeric aUPD. A. Total aUPD. B. MRC. MRC. MRC. MRC. MRC. GIST. GIST. GIST. GIST. GIST. EWS. EWS. EWS. EWS. EWS. aRMS. aRMS. aRMS. aRMS. aRMS. Leiomyosarcoma. Leiomyosarcoma. Leiomyosarcoma. Leiomyosarcoma. Leiomyosarcoma. D. C.

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Supplementary figure 2

Supplementary figure 2 PCA analysis of all the different cell lines used in the microarray experiments revealing three distinct sub groups ; translocation positive RMS cells (RMS, RMZ, RH3, RH40, RH41), translocation negative RMS cells (RD & RH18)and myoblasts (MYO & BIO).

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Supplementary Figure S7

A. MIM-R-GFP. 100. 80. 60. 40. Lung metastasis (14 days). 20. MIM-R-ILEI. 0. MIM-R -GFP. MIM-R -GFP -dnP. MIM-R -ILEI. MIM-R -ILEI -dnP. >100 cells. 50-100 cells. < 50 cells. Supplementary Figure S7. C Lahsnig et al. B. Metastases of different sizes (percent of total).

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Supplementary figure 3

1. 2. 3. 4. 5. 6. M. M. 1. 2. Probe: Unc5b. 7 kb. 28 s RNA. Supplementary figure 3. a. Specificity of the Unc5b splicing morpholino .

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Supplementary figure 1

Supplementary figure 1. Viable. Viable. Apoptotic. Apoptotic. Necrotic. Necrotic. 120. SKOV-3. 100. 80. 60. % total number of cells. 40. 20. 0. 0 hr. 24 hr. 48 hr. 72 hr. 96 hr. - 22 kDa. WB:. - 15 kDa. α Bid α G3PDH. - 37 kDa. Caov-4. SKOV-3. A. 120. Caov-4. 100.

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Supplementary Figure 3

Ctl sip300. p300. E2F1. Actin. Cisp (24h). Supplementary Figure 3. A. B. 25. NT. Cisp (24h). 20. Apoptosis (%). 15. 10. 5. 0. Ctl. sip300. Neutralization of p300 prevents E2F1 accumulation and apoptosis in response to cisplatin.

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SUPPLEMENTARY FIGURE 1

SUPPLEMENTARY FIGURE 1. A. Remove. Full length ErbB2. p185. p185. p95. p95. p95ErbB2. Whole. CEER. Enriched. Lysate. Lysate. B. pLXSN vector. p185 ERBB2. p110 ERBB2. p95m ERBB2.

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Supplementary Data Figure 1

A. B. Supplementary Data Figure 1 A, Binding of Alexa 546-CRP ( red ) to COS-7 cells expressing LOX-1, SR-A, CD36, SR-BI, and dectin-1. b -galactosidase (lacZ) was used as a negative control. The nuclei of the cells were counterstained with DAPI( blue ).

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Supplementary Figure 6

Supplementary Figure 6. B. A. HSC/MFs. MSC. a. a. Wound healing assay. Wound healing assay. **. **. **. **. #. #. #. cells invading the artificial wound. cells invading the artificial wound. #. ##. ##. ##. ##.

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Ruhanen et al., Supplementary Figure 3

Mt-1 (R2). Mt-2 (R2). dsRNA:. Sc. Sc. LIII. LIII. DNA ligase III. HSP 60. Ruhanen et al., Supplementary Figure 2. A. Control. r 0 cells. Gel image of double-stranded DNA arc under UV illuminator. B. Control. r 0 cells. Southern hybridisation.

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Figure S3

H1435. Vehicle. HGF. EGF. pMet. IP: c-Met. c-Met. c-Met. b -Actin. Figure S3. A549. Vehicle. HGF. EGF. pMet. IP: c-Met. c-Met. 1 2.8 9.2. 1 2.1 7.5. c-Met. Total Lysate. Total Lysate. b -Actin. 1 1.3 1.5. 1 1.1 1.8.

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Supplementary Figure 5

Supplementary Figure 5. A. B. MITOCHONDRIA. TOTAL. WT. S46A. WT. S46A. p53. RITA. –. +. +. –. WB: p53. % of apoptotic cells. α-amanitin. –. +. –. +. WB: VDAC. WB: HIPK2. WB: PCNA. WB: p53pS46. NT. RITA. WB: p53. WB: Actin. C. D. pLPC. Pin1-HA. MITOCHONDRIA. TOTAL.

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Supplementary Figure 2

Supplementary Figure 2. PDPN + cells. A. 90.8%. CAF 4. 37.6%. PDPN - cells. 0.44%. low. PDPN. high. B.

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Supplementary Figure 1

Supplementary Figure 1. Expansion of MSCs in 20% oxygen vs 5% oxygen. Supplementary Figure 2. Expansion of Kaneka-filtered cells in Flasks (P4) and the Quantum (P2). Supplementary Figure 3. Adipogenesis. Osteogenesis. Chondrogenesis.

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Supplementary Figure 3

Supplementary Figure 3. PELP1 Tg. Con. A. Phos MAPK. Con. PELP1 Tg. B. Phos AKT. Figure S3. Representative IHC images showing the status of phos -MAPK ( A )and phos -AKT ( B )in the mammary glands of 6-month-old control and MMTV-rtTA-TetO-PELP1 bitransgenic mice.

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