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CRIPSR-Cas9? PowerPoint Presentation
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CRIPSR-Cas9?

CRIPSR-Cas9?

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CRIPSR-Cas9?

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  1. CRISPR-Cas9? “CRISPR-Cas9 Plus”! The genomic project about animals and plants of CRISPR - Cas9 system guided by RNA, make a great change in biology. This technique is more convenient and more effective compared with other genetic engineering tools. Therefore, it was taken full advantage all over the world after a few years of its discovery. But it can’t be appropriate for every experiment. So those researchers tried to upgrade this system. They tried to do the same thing to the new CRISPR-Cas9 just likeApple did to his products every year, to make it more powerful and advanced compared to the original. They are researchers from Harvard Medical School researchers. The spCas9 variants can target sites in human and zebra fish genes more precisely, which is not modified by the original one. CRISPR-Cas9: Nowadays in mammalian systems, CRISPRs are applied as gene editing tools. The Cas9 bacterial enzyme and 20-nucleotide RNAmolecule make the CRISPR-Cas9 nucleases. The SpCas9 used in the lab usually come from bacteria Streptococcus pyogenes. NGG form is necessary in the process of PAM sequencing, and this makes great limit to the using of Cas9. Recently CD Genomics has also introduced its new CRIPSR Cas9 sequencing service. To overcome this shortage, The researchers set up an engineering system. This system will let them find different PAM sequences quickly with the ability of SpCas9.At the same time, they found a SpCas9 variants can reduce omissions during editing genetic. In addition, the researchers also found two smaller orthologous proteins in bacteria Streptococcus thermophiles and Staphylococcus aureus, Cas9 St1Cas9 and SaCas9 The corresponding author Joung of this article said that their new findings should help all the researchers use Cas9.And he thinks the most important significance of the study is that they first confirmed SpCas9 can be modified with the method of directional protein evolution. They believe that using a similar approach can also improve other characteristics of Cas9 enzyme to meet the needs of more. Massachusetts General Hospital has applied for patent for the SpCas9 in the article.