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“CRISPR genome editing” Precise gene regulation/modification using the simple CRISPR/Cas9 system

“CRISPR genome editing” Precise gene regulation/modification using the simple CRISPR/Cas9 system . Jason Kerkvliet , M.S. Research Associate II Hoppe lab. March 4 , 2014. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system.

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“CRISPR genome editing” Precise gene regulation/modification using the simple CRISPR/Cas9 system

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  1. “CRISPR genome editing”Precise gene regulation/modification using the simple CRISPR/Cas9 system Jason Kerkvliet, M.S. Research Associate II Hoppe lab March 4, 2014

  2. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system

  3. DNA repair mechanisms: gene editing efficiency is enhanced by creating breaks in DNA

  4. How does one use CRISPR? For NHEJ • CRISPR vector $65 • 4 (2 sgRNA sequences) oligos $40 • BbsI$70 • Total = $175

  5. How does one use CRISPR? For HDR

  6. CRISPR gRNA design tool crispr.mit.edu

  7. CRISPR/Cas9 can be used to edit more than one gene in parallel Wang et al., Cell, 2013

  8. Gene knock-down (siRNA) versus gene knock out Shalem et al., Science 2014

  9. Eliciting gene function using a CRISPR mediated genome screen Shalem et al., Science 2014

  10. http://www.addgene.org/CRISPR/

  11. http://www.addgene.org/CRISPR/plant/

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