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Optimization of Cell Culture in Bioreactors for Protein Production Studies

Explore the impact of different growth conditions on cell culture in bioreactors. Study focuses on gene expression and protein production. Utilization of controlled environments for enhanced results.

catherine-mccall
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Optimization of Cell Culture in Bioreactors for Protein Production Studies

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  1. Introdução: • Placas • Garrafas • Biorreatores

  2. Dentro das Garrafas • Maior numero de células • Maior qtd proteína • Menor controle das [] de o2 e ph • amonia

  3. 200 Genes reg depleção o2 • Prodsproteiasrec / stress da bac

  4. meios • Lb • Tb • Msm • eb

  5. biorreatores • Condiçoes controladas

  6. Discos silicone polielastomeros

  7. dialise

  8. glucose

  9. No trabalho: • 2 linhagens de e coli • 3 proteinas diferentes ( 3 plasmideos) • 4 meios • Garafas e placas

  10. eb

  11. Condições de cultivo

  12. Resultados:

  13. dicussão • Cneg do gel • Tempo • Questaoamp • Estoque ou placa

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