LIGHT ABSORPTION SPECTROSCOPY. colorimetric analysis of nmol samples of macromolecules Prof. Eric Wickstrom. E. B. orthogonal electronic (E) and magnetic (B) components of linearly polarized light. Prism Slit.
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colorimetric analysis of nmol samples of macromolecules
Prof. Eric Wickstrom
orthogonal electronic (E) and magnetic (B) components of linearly polarized light
I0 is the current measured by the photomultiplier tube when the light goes through the reference cuvet with buffer but not sample.
The most commonly used unit is the absorbance unit, AU, or OD in biochemical slang, an amount of a substance that will give an absorbance of 1.0 at the wavelength of interest in a cuvet with a pathlength of 1.0 cm, when dissolved in 1.0 mL of buffer.
1 mg DNA = 20 A260U
1 mg protein = 1 A280U
We write the relationship as A= εlc, where l is the pathlength in cm, and c is the concentration in mole/liter.