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pUC 18 3 kb

Rational behind Part A:. pUC 18 3 kb. Sal I. Sac I. Xba I. Hind III. Bam HI. Bam HI. Not drawn to scale. 1.5 Kb BamHI fragment Inserted Creating ICE-1 molecular size 4.5 Kb. Hind III. Hind III. Hind III. Hind III. Hind III. Hind III. Use guide lines or graph paper.

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pUC 18 3 kb

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  1. Rational behind Part A: pUC 18 3 kb Sal I Sac I Xba I Hind III Bam HI Bam HI Not drawn to scale 1.5 Kb BamHI fragment Inserted Creating ICE-1 molecular size 4.5 Kb

  2. Hind III Hind III Hind III Hind III Hind III Hind III Use guide lines or graph paper A) Scaled Map 1.5 Kb cDNA of ice fish 1,500 0 1,000 500 Mark on known vector sites Xba I Hind III Sac I Sal I Bam HI - 3000, 1500 Bam HI Bam HI Sac I - 3500, 1000 Sac I Sac I Xba I - 3750, 750 Xba I Xba I Sal I - 3600, 900 Sal I Sal I Sac I / Sal I - 3000, 600 500, 400 Sal I Sac I Sal I Sac I 200 400 400 Hind III - 3500, 400, 200 (sum 4100, either second 400 or 2 more 200. As both double digests have 400 band in addition to 300 and 100) Bands therefore must be two 400) Either Hind III Hind III Hind III Hind III 400 200 400 or Hind III Hind III Hind III Hind III 400 200 400 or Hind III SaI I (double digest 3500, 400, 300 200, 100) Sac I (double digest 3000, 500, 400, 300 200, 100) Sal I Xba I Hind III Sac I Sal I Xba I Sac I Composite

  3. Rational behind Part B: pUC 18 3 kb Sal I Sac I Sph I Pst I Pst I Not drawn to scale 7 Kb Pst I fragment of genomic DNA Sac I / Sal I - 3000, 600 500, 400 Sal I Sac I Sal I Sac I Sac I / Sal I 400 bp fragment

  4. Sph I Sal I Sa1 I Sa1 I Sa1 I Sa1 I Sa1 I Sa1 I Sa1 I Sa1 I Sa1 I Sa1 I Sa1 I Sa1 I 0 10 Kb 5 Kb B) Draw a scaled Map of the genomic clone 3 Kb vector 7 Kb Insert Mark on known vector sites Sph I Sal I Sac I Sph I - 10000 labeled 10000 Sph I labelled Pst I -7000, 3000 labeled 7000 Pst I labelled Pst I Sac I - 6000, 4000 labeled 6000 or labelled Sac I Sac I Either Sac I labelled Sac I / Sph I - 6000, 300, 1000 labeled 6000 labelled Sac I Sac I Sph I labelled SaI I - 5500, 3500, 1000 labeled 5000, 1000 labelled labelled Sal I labelled labelled 400 bp Sac I / Sal I fragment know taking up 2.5 Kb indicating the insertion of one or more intron with combined molecular weight of 2.1 Kb. Sac I/ SaI I - 4000, 3500, 1000, 1500 labeled 1500, 1000 Sac I Sac I Pst I labelled Pst I Composite Map Sac I Sac I

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