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Bioengineering the Future with Shell Oil

Bioengineering the Future with Shell Oil. Transport Fuels. 75% of liquid fuels are used for transport. 70% of that in cars. Retail fuel is 95% fossil alkanes and 5% from a biological source. By 2020, global demand for bio-derived fuels will be 500B litres. Wood Image.

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Bioengineering the Future with Shell Oil

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  1. Bioengineering the Future with Shell Oil

  2. Transport Fuels • 75% of liquid fuels are used for transport. 70% of that in cars. • Retail fuel is 95% fossil alkanes and 5% from a biological source. • By 2020, global demand for bio-derived fuels will be 500B litres. Wood Image

  3. The Exeter-Shell Projects Development of High-throughput Mutant Analysis and Wax Imaging Bacterial Population Sampling for HC Production Alkane Synthesis in Mycobacterium Inducible Wax Synthesis In Salt Cress Genes for Alkane Production in Higher Plants High-throughput Screen Development Rhodosporidium toruloides Genome Analysis Alkane Production & Secretion in Vibrio furnissii Forced Evolution & Mutagenesis for Increased FA Gene & Pathway Discovery HC Synthesis Screen in Oleaginous Yeasts Molecular & HC Analysis Of Isolated Mutants Molecular Bioinformatics HC Analysis Molecular Control of HC Production & Secretion in Botryococcus braunii Molecular Control of Hydrocarbon Synthesis & Secretion in Bacteria, Yeast and Algae for Advanced Biofuel Production Botryococcus braunii Culture Systems Productivity & HC Extraction B. Braunii Genome Evolution B. braunii-Bacterial Interactions Metabolism & Enzymes Culture Systems & DSP Alkane Synthesis in Desulfovibrio Synthetic Ecology for Optimum Algal Growth Alkane Synthesis in cyanobacteria Synthetic Biology C-Flux Engineering In Yeast FA Catabolism in cyanobacteria Variable Chain Length & Branched HC in Fermentative Microbes

  4. Synthetic Biology ILV MDHLA Keto acid bkdAA & bkdAB ilvE bkdB acoL Engineer the production of gasoline, jet-fuel, diesel and lubricant oil in E. coli that are identical to fossil fuels. Biological Alkanes require no alteration of current engine technologies. Acetyl CoA Malonyl-ACP Keto acyl CoA accA to accD fabH fabHB Fatty acyl-ACP Branched fatty acyl-ACP tesA luxD luxD FFA FATB1 FATB1 luxC & luxE F-al NpAD Alkanes & alkenes

  5. “Third Generation” Biofuels from Algae Renewable. Sustainable. Productive. CO2 Mitigation. Experimental.

  6. Botryococcus braunii Oils. Synthesises and secretes very long chain hydrocarbons. Major fossil component of oil wells.

  7. Botryococcus braunii Research. Genome sequencing and bioinformatics to elucidate oil biosynthetic pathways & interactions with symbiotic bacteria. Quantify B. braunii physiology for Life Cycle Analysis. Model and test oil production in industrial culture conditions.

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