enhancing the efficiency of a polymerase chain reaction using au nanoparticles l.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Enhancing the Efficiency of a Polymerase Chain Reaction Using Au Nanoparticles PowerPoint Presentation
Download Presentation
Enhancing the Efficiency of a Polymerase Chain Reaction Using Au Nanoparticles

Loading in 2 Seconds...

play fullscreen
1 / 7

Enhancing the Efficiency of a Polymerase Chain Reaction Using Au Nanoparticles - PowerPoint PPT Presentation


  • 236 Views
  • Uploaded on

Enhancing the Efficiency of a Polymerase Chain Reaction Using Au Nanoparticles The Goal of our studies

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

Enhancing the Efficiency of a Polymerase Chain Reaction Using Au Nanoparticles


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
the goal of our studies
The Goal of our studies
  • Nanoparticles show excellent heat transfer properties, and rapidly dissipate energy to their surroundings. These properties were used to enhance the efficiency of real-time polymerase chain reaction (real-time PCR), and PCR reaction.
  • Gold colloid with a diameter of 13nm was added into the polymerase chain reaction.
slide4

DNA fragment view

EGFP-N

GFP Sequences

EGFP-C

About 630bp

EGFP-N-S

GFP-IS

EGFP-N-AS

About 150bp

EGFP-N-S (5-TGCAGTGCTT -CAGCCGCTAC-3)

EGFP-N-AS (5’-CAGCTCGATGCGGTTCACCA-3’),

au nanoparticles were not changed after real time pcr
Au nanoparticles were not changed after real-time PCR.
  • Detection of PCR products before and after real-time PCR by UV-vis spectrometer. Both have absorbance in 520 nm, same as the spectrum in Figure 1B, representing the 13nm of gold colloid, a peak was detected.
  • (B) TEM image shows the Au nanoparticles were no change (13 nm) after real-time PCR.
the effect of au nanoparticles on pcr efficiency
The effect of Au nanoparticles on PCR efficiency

The positive control group was compared with the Case D group, in which 7.52×10-7 mM of gold particles were added. The reference PCR time used was denaturing/95C, 15s/, annealing/58C, 15s/, extending /72C, 30s. (A) The positive control group; (B) Case D group; (C) The reaction time was reduced to 2/3 of the reference PCR time. (D) The same as Fig. 4C, in case D the reaction time was reduced to 2/3 of the PCR reaction time. (E) The reaction time was reduced to 1/3 of the reference PCR time. (F) The PCR reaction time was reduced to 1/3 of Fig. 4D.