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The Polymerase Chain Reaction

The Polymerase Chain Reaction. Some milestones In molecular biology recognised by the award of the Nobel prize. Table (continued). Number of publications citing the polymerase chain reaction from 1985 to 1999. Klenow fragment used for DNA sequencing. Primers for DNA polymerase.

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The Polymerase Chain Reaction

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  1. The Polymerase Chain Reaction Some milestones In molecular biology recognised by the award of the Nobel prize

  2. Table (continued)

  3. Number of publications citing the polymerase chain reaction from 1985 to 1999

  4. Klenow fragment used for DNA sequencing

  5. Primers for DNA polymerase

  6. Primer design

  7. The use of PCR to amplify DNA moleculesin vitro

  8. The basic polymerase chain reaction

  9. The polymerase chain reaction

  10. PCR amplification of DNA Fragment

  11. The PCR cycle

  12. The PCR leads to amplification of the target sequences

  13. Mismatching primers can lead to the efficient amplification of undesired sequences

  14. Cloning DNA using the PCR and the consequences of polymerase mistakes

  15. Amplifying cloned DNA from vectors

  16. Alu-PCR is used to isolate human-specific DNA sequences

  17. Asymmetric PCR For producing single- stranded DNA for sequencing

  18. Detecting cancer cells using the PCR

  19. Determining sex of fetuses at risk for X-linked inherited disorders

  20. Nested Primers increase the specificity of a PCR

  21. Nested PCR

  22. Gene linkage analysis using single sperm

  23. Reverse transcriptase PCR (RT-PCR)

  24. Competitor RT-PCR

  25. Inverse PCR

  26. Some variants of the basic PCR Process

  27. Visualisation of PCR products

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