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Manual Tracking

An imagej plugin with cell tracking capabilities. Manual Tracking. Acquiring Manual Tracking. The first step to acquiring the manual tracking plugin is searching “ imagej cell tracking” and clicking the first link.

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Manual Tracking

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  1. An imagejplugin with cell tracking capabilities Manual Tracking

  2. Acquiring Manual Tracking • The first step to acquiring the manual tracking plugin is searching “imagej cell tracking” and clicking the first link. • Once on the ibidi page select “Manual Tracking” from the left menu containing Product Categories • From this page click the link shown below

  3. Acquiring Manual Tracking(cont.) • The next step is to download Manual Tracking from the purple link shown and move this file to the plugins folder of imagej

  4. Using Manual Tracking • Once in imagej, open the zvi file you wish to track. • Click the Plugins button and select Manual Tracking in the drop down menu • A new window should open. This window allows you to set the parameters for the track you are about to make. • The time interval and x/y calibration values can be selected here. A z value can also be calibrated if 3D tracking is being done.

  5. Using Manual Tracking • The Add track button in the top left should then be clicked. • Clicking on the nucleus will now produce xy points that pop up in a new window. If you continue to track the cell then the Manual Tracking program will produce distance and velocity values for the two points.

  6. Further Information • The two following links offer guidelines to doing other operations with the manual tracking plugin • http://rsb.info.nih.gov/ij/plugins/track/track.html • http://rsb.info.nih.gov/ij/plugins/track/Manual%20Tracking%20plugin.pdf

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