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Amino Sugar-N Soil Test. Jacob Bushong SOIL 5813 Feb. 14, 2014. Nitrogen Soil Test History. “Holy Grail” of soil test methods Several researchers have attempted Bremner , Stanford, Keeney Biological or Chemical Methods Incubation Acidic or alkaline oxidation or hydrolysis

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Amino sugar n soil test

Amino Sugar-N Soil Test

Jacob Bushong

SOIL 5813

Feb. 14, 2014


Nitrogen soil test history
Nitrogen Soil Test History

  • “Holy Grail” of soil test methods

  • Several researchers have attempted

    • Bremner, Stanford, Keeney

  • Biological or Chemical Methods

    • Incubation

    • Acidic or alkaline oxidation or hydrolysis

    • Analysis of salt extractions


Beginning of the isnt
Beginning of the ISNT

  • Dr. Mulvaney had noticed different responses to sidedress fertilizer applications in corn

  • Investigated the different organic N forms in soil

    • Total N

    • Acid hydrolyzable

      • NH4

      • Amino Sugar

      • Amino Acid


Hydrolyzable amino sugar n
Hydrolyzable Amino Sugar N

  • Mulvaney et al. (2001)


Development of isnt
Development of ISNT

  • Time needed to conduct the hydrolyzable soil test was greater than 12 hours.

  • Quicker/simpler methods were evaluated

  • Accelerated Diffusion


Isnt results
ISNT Results

  • Khan et al. (2001)


Nitrogen soil test for rice
Nitrogen Soil Test for Rice

Arkansas Delta Soils (n = 16)

United States Soils (n = 50)

  • Bushong et al. (2007)


Evaluation of hydrolyzable amino sugar n
Evaluation of Hydrolyzable Amino Sugar-N

  • Bushong et al. (2008)


Evaluation of hydrolyzable amino sugar n1
Evaluation of Hydrolyzable Amino Sugar-N

6 Hours

10 Minutes

  • Bushong et al. (2008)


Evaluation of hydrolyzable amino sugar n2
Evaluation of Hydrolyzable Amino Sugar-N

N-ST*R

  • Bushong et al. (2008)


Field evaluation of n st r
Field Evaluation of N-ST*R

  • Roberts et al. (2011)


Other s results for corn
Other’s Results for Corn

  • Laboski et al. (2008)