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Intron and Exon in Eukaryotic Cells PowerPoint Presentation
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Intron and Exon in Eukaryotic Cells

Intron and Exon in Eukaryotic Cells

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Intron and Exon in Eukaryotic Cells

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  1. Intron and Exon in Eukaryotic Cells exon exon exon intron intron 5’ 3’ 5’ 3’ DNA promotor stop codon start codon Transcription mRNA Processing cap poly A tail Splicing Intron deleted mature mRNA To cytoplasm Take place in nucleus Juang RH (2004) BCbasics

  2. cDNA Is Reverse Transcribed from mRNA 5’ 3’ 3’ 5’ 5’ Baltimore, Dulbecco, Temin (1975) mature mRNA poly A tail Reverse transcription TTTT 3’ 5’ RNA hydrolysis DNA polymerase 3’ CCC 5’ GGG 3’ Juang RH (2004) BCbasics

  3. Two Libraries: cDNA Library vs Genomic Library Genes in expression Total Gene Chromosomal DNA mRNA Reverse transcription Restriction digestion Complete gene cDNA Gene fragments Smaller Library Larger Library Vector: Plasmid or phage Vector: Plasmid Juang RH (2004) BCbasics