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Regulation of Microfilaments

Regulation of Microfilaments. Lecture 32B BSCI 420/421 Nov 15, ’02 “Where is the wisdom we have lost in knowledge? Where is the knowledge we have lost in information?” -T.S. Eliot The Rock 3. Actin Assembly and its Regulation 4. Actin-Associated proteins.

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Regulation of Microfilaments

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  1. Regulation of Microfilaments • Lecture 32B BSCI 420/421 Nov 15, ’02 • “Where is the wisdom we have lost in knowledge? • Where is the knowledge we have lost in information?” • -T.S. Eliot The Rock • 3. Actin Assembly and its Regulation • 4. Actin-Associated proteins

  2. Actin microfilaments show many of the same properties as MTs: 1. Nucleation, linear assembly, and steady state kinetics 2. Polar filaments 3. Treadmilling 4. Dynamic instability 5. Nucleotide splitting and exchange (ATP instead of GTP) 6. Regulation by associated proteins 1. Nucleation is aided by Actin Related Protein (ARP) complexes

  3. Regulation by associated proteins: a. Regulation of assembly

  4. b. Formation of filament arrays or microvillus

  5. Diff. Arrays by different actin cross-linking proteins: Two types of actin bundles: a-actinin

  6. Microvilli increase the surface area of many epithelial cells

  7. Microvilli are used for trapping prey by choanoflagellates and Sponges. Microvilli are sensory receptor organelles in invertebrate vision and in vertebrate hearing. Organ of Corti in cochlea. Tilting of mvi causes bundles to tilt opening mech-gated ion channels. Fig. 22-12 &13.

  8. Actin binding to membranes can be reg by P04lation of ERM (ezrin, radixin, or moesin) or other membrane binding proteins.

  9. Effect of Rac, Rho, and Cdc42 activation on the actin organization of fibroblasts.(actin stained w fluorescent phalloidin, FCs w a-vinc. (Rac, rho, & Cdc42 are all monomeric G-proteins that rec &convey signals from extra-cell receptors

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