liquid based cytology l.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
LIQUID BASED CYTOLOGY PowerPoint Presentation
Download Presentation
LIQUID BASED CYTOLOGY

Loading in 2 Seconds...

play fullscreen
1 / 15

LIQUID BASED CYTOLOGY - PowerPoint PPT Presentation


  • 499 Views
  • Uploaded on

LIQUID BASED CYTOLOGY. Irene M le Roux Programme Head / National Cytology coordinator NHLS. NHLS STUDIES. Two separate studies: 2006 at the Tygerberg laboratory 2008 at the Braamfontein laboratory . Reasons for investigating LBC:. New Technology Improvement in screening volumes

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'LIQUID BASED CYTOLOGY' - auberta


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
liquid based cytology

LIQUID BASED CYTOLOGY

Irene M le Roux

Programme Head / National Cytology coordinator

NHLS

nhls studies
NHLS STUDIES

Two separate studies:

  • 2006 at the Tygerberg laboratory
  • 2008 at the Braamfontein laboratory
reasons for investigating lbc
Reasons for investigating LBC:
  • New Technology
  • Improvement in screening volumes
  • Improvement in diagnosis
  • Improvement in unsuitable rates
  • Improvement in adequacy rates
  • Improvement of TAT
for the study
For the study:
  • Invited the 4 LBC companies in SA to participate
  • Collection of material was done according to an approved protocol
  • The CEVEX brush was used
  • The cervical (PAP) smear was made first
  • The remaining material rinsed in the preservative
  • The head of the brush was placed in the vial
preparation of the specimens
Preparation of the specimens
  • A national Cytology workshop was held
  • Presentations & practical demonstrations by each company
  • The PAP smears formed part of the routine work
  • The LBC prepared smears were screened “blind”
  • The results were compiled by an independent person
  • One company withdrew shortly before the workshop
preparation
PREPARATION
  • Representatives from the companies prepared the collected samples as part of the demonstrations
results 1
Results (1)
  • A total of 373 cases were included
  • LBC showed an improvement by:
    • lowering the ASCUS rates

(4.3 vs 6.4%)

    • increasing the LSIL rates

(13.4 vs 12.1%)

    • lowering the unsatisfactory rates

(1.3 vs 1.9%)

results 2
Results (2)
  • LBC and the PAP smears were equal on the:
    • HSIL (5.6% each)
  • The PAP smears had more Endo -cervical component present

(85.7 vs 79.2 %)

second nhls study
SECOND NHLS STUDY
  • The 2008 study was a collaboration between the Braamfontein laboratory & SurePath.
  • More than 1000 cases in study
  • Preliminary results:
      • Improvement of ±3% on LSIL cases
      • ±0.8% improvement on unsatisfactory cases
conclusion
CONCLUSION
  • The advantages of LBC in 2 studies:
    • Improvement in LSIL & unsatisfactory rates
    • Smaller surface to be screened
    • Monolayer preparations vs thick smears
    • Improved TAT
    • Less exudate and blood that obscure cells
conclusion 2
CONCLUSION (2)
  • The disadvantages:
    • Cost