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CMV promoter

A. sense strand. loop. antisense strand. Bgl II site. Sfi I site. 5’-GATCT GTG GAA GAT TCA GCC AAG C TTCAAGAGA G CTT GGC TGA ATC TTC CAC TTT TTT GGCCACGT- 3’ 3’- A CAC CTT CTA AGT CGG TTC G AAGTTCTCT C GAA CCG ACT TAG AAG GTG AAA AAA CCGGT - 5’. B. AR. U6 promoter.

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CMV promoter

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  1. A sense strand loop antisense strand Bgl II site Sfi I site 5’-GATCT GTG GAA GAT TCA GCC AAG C TTCAAGAGA G CTT GGC TGA ATC TTC CAC TTT TTT GGCCACGT- 3’ 3’- A CAC CTT CTA AGT CGG TTC G AAGTTCTCT C GAA CCG ACT TAG AAG GTG AAA AAA CCGGT - 5’ B AR U6 promoter CMV promoter pUC ori IRES Amp(R) pSiAR-EGFP 5440 bp EGFP SV40 poly A

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