Sequence Results. T he A rabidopsis I nformation R esource. The National Center for Genome Resources (NCGR), Santa Fe, New Mexico The Arabidopsis Biological Resource Center (ABRC), Ohio State University Carnegie Institution, Department of Plant Biology, Stanford University .
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The National Center for Genome Resources (NCGR), Santa Fe, New Mexico
The Arabidopsis Biological Resource Center (ABRC), Ohio State University
Carnegie Institution, Department of Plant Biology, Stanford University
Mission Summary: Provide research resources for the
Arabidopsis research community
To determine the function of all genes in
Arabidopsis thaliana by the year 2010
• Gene Overview
mitochondria or in the endoplasmic reticulum of cells where they metabolize
thousands of endogenous and exogenous compounds.
At (Arabidopsis thaliana)
1,2,3,4,5 (chromosome number) or M for mitochondrial or C for chloroplast.
g (gene), other letters possible for repeats etc.)
12300 (five-digit code, numbered from top/north to bottom/south of chromosome)
A type I topoisomerase from P1 bacteriophage that catalyzes site-specific
recombination of DNA between loxP sites
The loxP recognition element:
Transform in DH5α.Select on Amp.
Qiagen miniprep DNA, approx. 200 ng/µl
Recommended reaction for Cre-LoxP
Green Fluorescent Protein
X-Gluc + β-Glucuronidase =
General localization and
Evaluation of gene expression/induction.
The Arabidopsis Information Resource
• The primary goal of TAIR is to assist the Arabidopsis community
discover gene function.
• Extensive gene knockout and localization tools (T-DNA lines, full-length cDNAs,
and pUNI cDNAs) serve as the core elements of what TAIR offers.
• The TAIR website, its databases, and resources continue to expand, so
if you are unable to find a T-DNA insertion today…try tomorrow!
Syngenta Inc has now donated about 9,000 more lines to ABRC and
sequences to this site at Mar. 31 2006
Cre-loxP recombination vectors for the expression of Riken Arabidopsis full-length cDNAs in plants Toshiro Shigaki, Mamata Kole, John M. Ward, Heven Sze, and Kendal D. Hirschi
BioTechniques Vol. 39, No. 3: pp 301-303 (Sep 2005)
The Cre-loxP recombination-based reporter system for plant transcriptional expression studies
Shigaki, T., R. Ravindranadha, A.B. Sivitz, J.M. Ward, H. Sze, and K.D. Hirschi
Plant Mol. Biol. 58:65-73 (2005)
The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without
Liu, Q., M.Z. Li, D. Leibham, D. Cortez, and S.J. Elledge
Curr. Biol. 8:1300-1309 (1998)
A structural view of Cre-loxP site-specific recombination
Gregory D. Van Duyne
Annual Review of Biophysics and Biomolecular Structure Vol. 30: 87-104 (2001)