1 / 1

Investigating the Effects of Taxol on MCF-7 Cell Cycle Progression and PARP Cleavage

This study examines how Taxol (3 ng/ml) influences cell cycle phases and apoptosis in MCF-7 breast cancer cells using cleaved PARP as a marker. By analyzing the impact of siRNA targeting cyclin B1 and control siGFP in a 40-cell well format, we explore the transition of cells through G2/M and G0/G1 phases. The findings contribute to understanding the molecular mechanisms by which Taxol induces cell death and cell cycle arrest, highlighting the roles of cyclin B1 and PARP in cancer treatments.

anika-morris
Download Presentation

Investigating the Effects of Taxol on MCF-7 Cell Cycle Progression and PARP Cleavage

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Fig. 4 A cyclin B1 β-actin siRNA3+ siGFP+ C+ PARP - - - + + + taxol (3 ng/ml) cleaved PARP B siRNA3 siGFP C 40 MCF-7 30 20 cells/well  10 C 4 10 0 C siGFP siRNA3 C+ siGFP+ siRNA3+ S G2/M 75 G0/G1 50 % 25 0 C siGFP siRNA3 C+ siGFP+ siRNA3+

More Related