1 / 34

Fig. 4-1

Chapter 4 overview. Fig. 4-1. Genetic recombination : mixing of genes during gametogenesis that produces gametes with combinations of genes that are different from the combinations received from parents. Independent assortment of homologous chromosomes (Anaphase I). Genes on non-

tadita
Download Presentation

Fig. 4-1

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Chapter 4 overview Fig. 4-1

  2. Genetic recombination: mixing of genes during • gametogenesis that produces gametes with • combinations of genes that are different from • the combinations received from parents. • Independent assortment of homologous • chromosomes (Anaphase I). Genes on non- • homologous chromosomes (unlinked genes) • assort independently.

  3. Fig. 4-6

  4. Using a testcross to distinguish gamete genotypes Fig. 4-7

  5. 50% = independent assortment (genes are not linked) Fig. 4-8

  6. Genetic recombination: mixing of genes during • gametogenesis that produces gametes with • combinations of genes that are different from • the combinations received from parents. • Independent assortment of homologous • chromosomes (Anaphase I). Genes on non- • homologous chromosomes (unlinked genes) • assort independently. • Crossing over (recombination among linked • genes)

  7. cis linked: both dominant alleles on the same homolog trans linked: dominant alleles on different homologs Fig. 4-2

  8. Fig. 4-3

  9. Crossing over • Physical exchanges among non-sister chromatids; • visualized cytologically as chiasmata • Typically, several crossing over events occur within • each tetrad in each meiosis (chiasmata physically • hold homologous chromosome together and assure • proper segregation at Anaphase I) p. 115

  10. Crossing over occurs at the four-strand stage (pre-meiotic G2 or very early prophase I) Fig. 4-4

  11. Crossing over can involve 2, 3, or 4 chromatids in a single meiosis Fig. 4-5

  12. Crossing over • Physical exchanges among non-sister chromatids; • visualized cytologically as chiasmata • Typically, several crossing over events occur within • each tetrad in each meiosis (chiasmata physically • hold homologous chromosome together and assure • proper segregation at Anaphase I) • The sites at which crossing over occur are random • The likelihood that a crossover occurs between any • two particular sites (genes) is a function of the • physical distance between those two sites

  13. Crossing over usually affects a minority of chromatids in a collection of meioses – recombinants are typically a minority of products Fig. 4-9

  14. <50% = linked genes Fig. 4-10

  15. A.H. Sturtevant (1911-3): frequency of crossing over between two genes is a function of their distance apart on the chromosome; created the first genetic map number of recombinants Recombination frequency = total number of progeny One map unit = one centimorgan = 1% recombinants

  16. Rationales: • Crossover events are random • Greater separation, greater likelihood that crossover will occur • Map distance should be sum of smaller intervals • Construct entire chromosome maps by mapping intervals • Linear map correlates with linear chromosome Fig. 4-11

  17. Markers used in trihybrid testcross in Drosophila v = vermilion eyes (red eyes; v+are red-brown) cv = crossveinless (cv+ wings have crossveins) ct = cut wing (ct+ wings have regular margins)

  18. Data from three-point testcross v+/ v cv+/ cv ct+/ ct X v / v cv / cv ct / ct (trihybrid) (tester) Progeny phenotypes v cv+ ct+ 580 v+ cv ct 592 v cv ct+ 45 v+ cv+ ct 40 v cv ct 89 v+ cv+ ct+ 94 v cv+ ct 3 v+ cv ct+ 5 1448

  19. Steps in solving three-point testcross problem • Anticipate and identify eight types of products (23) • Identify pairs of reciprocal products

  20. Data from three-point testcross v+/ v cv+/ cv ct+/ ct X v / v cv / cv ct / ct (trihybrid) (tester) Progeny phenotypes v cv+ ct+ 580 v+ cv ct 592 v cv ct+ 45 v+ cv+ ct 40 v cv ct 89 v+ cv+ ct+ 94 v cv+ ct 3 v+ cv ct+ 5 1448

  21. Steps in solving three-point testcross problem • Anticipate and identify eight types of products (23) • Identify pairs of reciprocal products • Identify parental types as the most frequent pair of • products • Identify double crossover products as least frequent • pair of products

  22. Data from three-point testcross v+/ v cv+/ cv ct+/ ct X v / v cv / cv ct / ct (trihybrid) (tester) Progeny phenotypes v cv+ ct+ 580 v+ cv ct 592 v cv ct+ 45 v+ cv+ ct 40 v cv ct 89 v+ cv+ ct+ 94 v cv+ ct 3 v+ cv ct+ 5 1448 Parental types - nco sco sco dco

  23. Steps in solving three-point testcross problem • Anticipate and identify eight types of products (23) • Identify pairs of reciprocal products • Identify parental types as the most frequent pair of • products • Identify double crossover products as least frequent • pair of products • Compare the parental and double crossover products • to deduce the order of the three gene loci

  24. Fig. 4-12 In dco products, the central marker is displaced relative to the parental types

  25. Fig. 4-13

  26. Steps in solving three-point testcross problem • Anticipate and identify eight types of products (23) • Identify pairs of reciprocal products • Identify parental types as the most frequent pair of • products • Identify double crossover products as least frequent • pair of products • Compare the parental and double crossover products • to deduce the order of the three gene loci • Compute map distances by breaking down the • results for each interval

  27. Fig. 4-12 85 + 8 1448 (0.064) 183 + 8 1448 (0.132) RF =

  28. Fig. 4-12 85 + 8 1448 (0.064) 183 + 8 1448 (0.132) RF = 13.2 m.u. 6.4 m.u. v ct cv

  29. Interference: crossing over in one region interferes with simultaneous crossing over in adjacent regions Expected frequency of dco = product of frequency crossovers in two regions 0.132 X 0.064 = 0.0084 0.084 X 1448 = 12 expected (if two sco are independent events)

  30. Interference: crossing over in one region interferes with simultaneous crossing over in adjacent regions Expected frequency of dco = product of frequency crossovers in two regions 0.132 X 0.064 = 0.0084 0.084 X 1448 = 12 expected (if two sco are independent events) Coefficient of coincidence = observed dco / expected dco 8 / 12 = 0.667 Interference = 1 – coefficient of coincidence 1 – 0.667 = 0.333

  31. Fig. 4-14 Tomato karyotype (n=12)

  32. Tomato linkage map (1952) Fig. 4-14

  33. Typical phenotypic ratios for a variety of crosses (complete allele dominance) p. 136

More Related