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Comprehensive Study on Neurogenesis and Apoptosis in Chronic Stress Model Using Immunohistochemical Analysis

This project aims to investigate neurogenesis and apoptosis in a chronic mild stress model using immunohistochemical analysis, focusing on brain regions like the hippocampus, amygdala, and prefrontal cortex. The study involves team workpackages for brain dissection, pharmacological treatments, gene expression analysis, and PC12α2 cell differentiation assessment. Methods include qRT-PCR, Western blotting, in situ hybridization, and manipulation of β-arrestins, Nrf2, and CREB.

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Comprehensive Study on Neurogenesis and Apoptosis in Chronic Stress Model Using Immunohistochemical Analysis

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  1. Project workflow Behavioral tests Immunohistochemical analysis of neurogenesis Chronic mild stress (uCMS) Animal sacrifice 1st Team Workpackage #1 Dissection of brain regions 3 wks Pharmacological treatments 4 wks BrdU Monoamines determination Plasticity related gene expression( qRT-PCR ) Immunohistochemical analysis of neuroprotection/apoptosis with in situ hybridization Hippocampus, amygdala, prefrontal cortex , anterior cingulate cortex , hypothalamus, parietal, temporal lobes 2nd Team Workpackage #2 Analysis of apoptosis with in situ hybridization Knock-down or overexpression of β-arrestins 3rdTeam Workpackage #3 PC12α2cells Analysis of neuronal differentiation, apoptosis and signal transduction Hippocampus, amygdala, prefrontal cortex , acc , hypothalamus Plasticity related gene expression (Western blotting) Dominant negative mutant or overexpression ofNrf2 & CREB

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