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CEREBROSPINAL FLUID. CSF FUNCTIONS. 1. Supply Nutrients 2. Remove Metabolic Waste 3. Protect and Cushion Brain and Spinal Chord. CSF Volume. Adults 140 to 170 mL Neonates 10 to 60 mL Located in the subarachnoid space between the arachnoid matter and pia matter.

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Presentation Transcript
csf functions
CSF FUNCTIONS
  • 1. Supply Nutrients
  • 2. Remove Metabolic Waste
  • 3. Protect and Cushion Brain and Spinal Chord
csf volume
CSF Volume
  • Adults 140 to 170 mL
  • Neonates 10 to 60 mL
  • Located in the subarachnoid space between the arachnoid matter and pia matter.
  • 20 mL produced per hour
csf production
CSF Production
  • Produced in the Choroid Plexus of brain
  • DOES NOT resemble ultrafiltrate of plasma
  • Large molecules are restricted
  • Blood Brain Barrier
csf collection
CSF Collection
  • Lumbar puncture
  • Tube 1 Chemistry and Serological testingFreeze
  • Tube 2 Microbiology RT
  • Tube 3 Hematology Refrigerate
  • STAT
  • DO NOT Discard Supernatant
csf appearance
CSF Appearance
  • Clear and Colorless – Normal
  • Cloudy or Turbid Protein, Cells Lipids
  • Xanthochromic pink, orange or yellow due to RBC degradation products
traumatic tap
Traumatic Tap
  • Uneven distribution of Blood
  • Clot Formation
  • Xanthochromic Supernatant
chemistry tests
Chemistry Tests
  • Protein 15 – 45 mg/dL
  • Protein electrophoresis
  • Glucose 2/3 of blood glucose
  • Lactate
  • Glutamine 8 – 18 mgdL
  • Enzymes LD CK-BB , 17 mg/dL
chemistry tests1
Chemistry Tests
  • Protein 15 – 45 mg/dL
  • Methods Turbidometric or Dye Binding
  • Turbidometric TCA or SSA
  • Dye Binding Coomassi Brilliant Blue
  • Electrophoresis