disposable molecular diagnostics microfluidic laboratories for the field n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Disposable molecular diagnostics: Microfluidic laboratories for the field PowerPoint Presentation
Download Presentation
Disposable molecular diagnostics: Microfluidic laboratories for the field

Loading in 2 Seconds...

play fullscreen
1 / 18

Disposable molecular diagnostics: Microfluidic laboratories for the field - PowerPoint PPT Presentation


  • 127 Views
  • Uploaded on

Disposable molecular diagnostics: Microfluidic laboratories for the field. Catherine Klapperich, Ph.D. Biomedical Microdevices and Microenvironments Laboratory www.klapperichlab.org Biomedical and Manufacturing Engineering Departments Boston University 3 October 2006.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Disposable molecular diagnostics: Microfluidic laboratories for the field' - aileen-rodriguez


Download Now An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
disposable molecular diagnostics microfluidic laboratories for the field

Disposable molecular diagnostics: Microfluidic laboratories for the field

Catherine Klapperich, Ph.D.

Biomedical Microdevices and Microenvironments Laboratory

www.klapperichlab.org

Biomedical and Manufacturing Engineering Departments

Boston University

3 October 2006

microfluidics applications
Microfluidics Applications
  • Diagnostics/Management
    • Point of Care
    • Disease Surveillance
  • Detection
    • Homeland Security
    • Fighting Force Protection
  • High Throughput Screening
    • Drug Discovery/Development
    • Cell Based Assays
    • Research Bench Applications
  • Micro-Reactions
    • Combinatorial Methods
  • Living Tissue Arrays
    • Drug Development
global impact
Case finding

Case management

Surveillance

24% of the current burden of disease could be averted if 80% of the population of low income countries received the following: prenatal/delivery care, family planning, treatment of TB, management of sick children and case management of STDs.

Implementation would cost $8/person per year.

Global Impact

Nature Reviews Microbiology2, 231-240 (2004) DIAGNOSTICS FOR THE DEVELOPING WORLD

state of the art
State of the Art
  • Microscopy
    • Parasitic and mycobacterial infections
    • Requires well trained technician
  • Cell Culture
  • EIA
  • Nucleic acid amplification

All require specialized equipment and/or technicians.

slide5

MicroTAS for Diagnostics

  • Sample introduction
  • Cell sorting/separation
  • Mixing
  • Lysis
  • Separation/concentration
  • Detection
  • Waste stream capture

Sample

Preparation

Fluidics

Detection

Input

Output

Control and Signal Processing

system schematic
System Schematic

Detection

Surfaces/Channels

Antibodies

Oligos

PCR

device design constraints
Device Design Constraints
  • Inexpensive materials
  • Rapid prototyping
  • Scale up/mass production
  • Shelf life of 1 year or more
  • Ease of use
  • On-board reagents
  • Disposable
  • Little sample preparation off chip
  • Low power or no power
materials requirements
Materials Requirements
  • Optical properties
    • UV transparent (for quantifying proteins, DNA and RNA)
    • Transparent to excitation and detection wavelengths (488 nm, FITC)
  • Thermal properties
    • For PCR (95 degrees Celsius)
    • For dimensional stability
  • Surface chemistry
    • Hydrophilic/hydrophobic
    • Non-binding
    • Binds specific molecules
    • Shelf life issues
slide9

Engineering Polymers for Microfluidic

Diagnostic Devices

PMMA

ZEONEX and ZEONOR by ZEON

Tg= 85-105C

Zeonor 750R, Tg 70C

Zeonex 690R Tg 136C

Ring Opening

Polymerization

Polycarbonate

Tg= 140-150C

ZEON Polymers are obtained by ring-opening metathesis polymerization (ROMP) of norbornene derivatives monomers followed by complete hydrogenation of double bonds.

Polystyrene

TOPAS by TICONA and APEL by Mitsui

Tg= 90-110C

Addition

Polymerization

R1,R2,R3; H

advantages of thermoplastic chips
Advantages of Thermoplastic Chips
  • Feature size is identical to PDMS but with long term dimensional stability.
  • Surface treatments are robust and do not “age” as on PDMS devices.
  • Permeability is low.
  • Thermoplastics can be purchased in grades that are certified non-pyrogenic (do not contain DNA or RNA destructive enzymes).
  • The per device material cost is low.
  • The plastic chips can be easily manufactured in-house using rapid prototyping techniques in production materials to test and optimize new chip layouts and chemistries quickly.
  • Internal structures (filters, valves, detection patches) can be fabricated in situ by light-directed processes.
  • Acrylics and cyclic polyolefins have low autofluorescence for high detection signal to noise ratios.
  • Acrylics and cyclic polyolefins are transparent to UV, which enables light directed processing of internal structures and UV detection of nucleic acid concentrations and integrity through the chip.
rapid prototyping
Rapid Prototyping
  • A cyclic polyolefin (Zeonex 690R) was used as chip material
  • Microchip fabricated by hot-embossing with a silicon master

Photoresist

Pressure and

Heat Applied

Si Wafer

Polymer

pellets

UV light

Mask

Embossed

substrate

DRIE

Thermally

bonded channels

slide12

Completed

fluidic

card

Glass or Si wafer

  • Photoresist coat
  • Mask, expose, develop photoresist
  • Etch glass
  • Remove photoresist

Etched glass plate

Electroplate

Plastic

cover

layer

Separate

Cover

and

seal

Metal electroform

Mold or emboss

Repeat

1000’s of times

Molded plastic card

Separate

Scale-up Fabrication

moving fluid
Moving Fluid
  • Pressure
  • Vacuum
  • Electroosmotic Flow
    • Surface Chemistry of Channels
    • Simultaneous Assay and Device Development
slide18

Jessica Kaufman

Arpita Bhattacharyya

Justyn Jaworski

Nathan Spencer

Dominika Kulinski

Dave Altman

Amy VanHove

Dr. Cassandra Noack

Coulter Foundation

Whitaker Foundation

CIMIT

NSF MUE

Pria Diagnostics, Inc.