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國立台灣大學生物資源暨農業學院 植物光生態生理學 (Plant Photo-ecophysiology) 第 7 章 ATP 的合成 楊棋明 博士 中央研究院植物研究所暨生物多樣性研究中心

國立台灣大學生物資源暨農業學院 植物光生態生理學 (Plant Photo-ecophysiology) 第 7 章 ATP 的合成 楊棋明 博士 中央研究院植物研究所暨生物多樣性研究中心 Email: cmyang@gate.sinica.edu.tw Tel: 02-27821258#612, 613. light. light. Thylakoid membrane. light. Stroma. Four stages of photosynthesis in higher plants. Stage 1: absorption of light

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國立台灣大學生物資源暨農業學院 植物光生態生理學 (Plant Photo-ecophysiology) 第 7 章 ATP 的合成 楊棋明 博士 中央研究院植物研究所暨生物多樣性研究中心

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  1. 國立台灣大學生物資源暨農業學院 植物光生態生理學 (Plant Photo-ecophysiology) 第7章 ATP的合成 楊棋明 博士 中央研究院植物研究所暨生物多樣性研究中心 Email: cmyang@gate.sinica.edu.tw Tel: 02-27821258#612, 613

  2. light light Thylakoid membrane light Stroma Four stages of photosynthesis in higher plants Stage 1: absorption of light Chl Chl* 2H2O O2＋4H＋＋4e－ Stage 2: electron transport and generation of a proton-motive force (pmf)/pH gradient 2H2O＋2NADP＋2H＋＋2NADPH＋O2 Stage 3: synthesis of ATP ADP3－＋Pi2－ ATP4－ Stage 4: carbon fixation 6CO2＋18ATP4－＋12NADPH＋12H2O C6H2O＋18ADP3－＋18Pi2－＋12NADP＋＋6H＋

  3. ATP＋H2O ADP＋Pi＋ 7.3kcal/mol ADP＋H2O AMP＋Pi＋7.3kcal/mol AMP＋H2O Adenosine＋Pi＋3.4kcal/mol Adenine 7.3kcal/mol 7.3kcal/mol 3.4kcal/mol Ribose Adenosine AMP ADP ATP Chemical structure of ATP, ADP and AMP and their energy

  4. ATP synthesis in chloroplast (1):the chemiosmotic hypothesis Pete Mitchell (UK) in 1961 proposed a chemiosmotic hypothesis to account for ATP formation accompanying electron transport in mitochondria and chloroplasts. Pete Mitchell was awarded the Nobel Prize in 1978. Paul Boyer (USA) and John Walker (UK), elucidating the ATPase structure and function, were awarded the Nobel Prize in 1997. The Chemiosmotic Hypothesis: Intact membranes are almost impermeable to the passive flow of protons. The electron donors and acceptors of electron and proton are arranged vectorially in the membrane. During photosynthetic electron transport, protons are translocated from the external medium (stroma) into the osmotic space of the intra-thylakoid membrane.

  5. ATP synthesis in chloroplast (2): the chemiosmotic hypothesis

  6. ATP synthesis in chloroplast (3): ATPase or ATP synthase/ coupling factors/ CF0CF1 complex Paul Boyer (USA) and John Walker (UK), elucidating the ATPase structure and function, were awarded the Nobel Prize in 1997.

  7. Inside-out and outside-in of mitochondrial and chloroplast

  8. Construction of artificial ATP synthesis system lumen

  9. ATP synthesis (4): bacteria, mitochondria, chloroplast

  10. Comparison of chemiosmosis in mitochondria and chloroplasts

  11. Subunits and their structures of ATPase complex in eukaryotes ★ (α3β3γδε) C, chloroplast N, nucleus

  12. ATPase in the bacteria, mitochondria, chloroplast

  13. Rotational model for ATP synthesis ★ ★ ★ ★ ★ ★

  14. Experimental evidence for rotation of ATPase ATPase is a rotatory motor.

  15. Mitochondrial ATPase

  16. Structure and location of ATPase on the thylakoid membrane

  17. Have you a nice day

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