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THE LIGHT MICROSCOPE AND THE ELECTRON MICROSCOPE. © 2007 Paul Billiet ODWS. UNITS OF MEASUREMENT. 1m = 10 3 mm (millimetres) 1m = 10 6 µm (micrometres) 1m = 10 9 nm (nanometres) Sometimes in old texts Angstroms (Å) are used (the diameter of a hydrogen atom) 1m = 10 10 Å .

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THE LIGHT MICROSCOPE AND THE ELECTRON MICROSCOPE


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units of measurement
UNITS OF MEASUREMENT

1m = 103 mm (millimetres)

1m = 106 µm (micrometres)

1m = 109 nm (nanometres)

Sometimes in old texts Angstroms (Å) are used (the diameter of a hydrogen atom)

1m = 1010 Å

© 2007 Paul Billiet ODWS

the electromagnetic spectrum
The electromagnetic spectrum

© 2007 Paul Billiet ODWS

light v electron
Light v Electron

© 2007 Paul Billiet ODWS

magnifying using the light microscope
Magnifying using the Light Microscope

© 2007 Paul Billiet ODWS

the light microscope v the electron microscope

FEATURE

LIGHT MICROSCOPE

ELECTRON MICROSCOPE

Electromagnetic spectrum used

Visible light

760nm (red) – 390nm Colours visible

Electrons

app. 4nm

Monochrome

Maximum resolving power

app. 200nm

0.2nm

Fine detail

Maximum magnification

x1000 – x1500

x500 000

Radiation source

Tungsten or quartz halogen lamp

High voltage (50kV) tungsten lamp

Lenses

Glass

Magnets

Interior

Air-filled

Vacuum

Focussing screen

Human eye (retina), photographic film

fluorescent (TV) screen,

photographic film

THE LIGHT MICROSCOPE v THE ELECTRON MICROSCOPE

© 2007 Paul Billiet ODWS

the light microscope v the electron microscope8

FEATURE

LIGHT MICROSCOPE

ELECTRON MICROSCOPE

Preparation of specimens

Temporary mounts living or dead

Tissues must be dehydrated

= dead

Fixation

Alcohol

OsO4 or KMnO4

Embedding

Wax

Resin

Sectioning

Hand or microtome

slices  20 000nm

Whole cells visible

Microtome only.

Slices  50nm

Parts of cells visible

Stains

Water soluble dyes

Heavy metals

Support

Glass slide

Copper grid

THE LIGHT MICROSCOPE v THE ELECTRON MICROSCOPE

© 2007 Paul Billiet ODWS

embedding in resin
Embedding in resin

© 2007 Paul Billiet ODWS

microtome knife
Microtome knife

© 2007 Paul Billiet ODWS

copper grid slides
Copper grid slides

© 2007 Paul Billiet ODWS

cell structure and microscopy13
CELL STRUCTURE AND MICROSCOPY

© 2007 Paul Billiet ODWS