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Lecture40&41 Edible Vaccines. EDIBLE VACCINES FROM PLANTS. Two strategies for production 1) Expression of foreign antigens in plant via stable transformation 2) Delivery of vaccine epitopes via plant virus (Mason and Arntzen, 1995).

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lecture40 41 edible vaccines

Lecture40&41Edible Vaccines

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slide2

EDIBLE VACCINES FROM PLANTS

Twostrategies for production

1) Expression of foreign antigens in plant via stable transformation

2) Delivery of vaccine epitopes via plant virus

(Mason and Arntzen, 1995)

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slide3

Strategies for the production of candidate vaccine

  • antigens in plant tissues
  • (Mason and Arntzen, 1995)

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slide4

HEPATITIS B SURFACE ANTIGEN IN TOBACCO

  • Gene encoding HBsAg expressed in tobacco plants
  • Targeted to the chloroplast
  • Genetic constructs-
  • 1) driven by CaMV 35S promoter
  • 2) 5' UTR from TEV and TMV
  • 3) signal peptide from soybean
  • VSP
  • 4) A plastid transit peptide

Contd...

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slide5

Chloroplast of tobacco

Contd...

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slide6

Structures of expression cassettes

Contd...

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Various expression vectors were constructed

  • pHB110 was plastid targeted
  • Expression level 0.01% of soluble protein
  • (Richter et al., 2000)

Contd...

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RNA Analyzed by Northern Blot Hybridized with

HBsAg coding sequence

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RABIES VIRUS G PROTEIN IN TOMATO

  • Gene linked to CaMV35S promoter
  • Introduced to tomato plants by Agrobacterium- mediated transformation
  • Expression of recombinant glycoprotein in leaves and fruits
  • Protein localized in golgi bodies, vesicles and plasmalemma

(Mc Garvey et al.,1995)

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slide10

Norwalk virus (cold virus) capsid protein in potato and tobacco

  • Causative agent for acute epidemic gastroenteritis
  • NVCP was fused to CaMV35S or patatin promoter
  • Transformation by Agrobacterium
  • Expression level:
  • 0.37% TSP in potato tubers
  • 0.23% TSP in tobacco leaves
  • (Mason et al., 1996)

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