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URIMEM, will facilitate biomarker discovery and change the face of medicine

URIMEM, will facilitate biomarker discovery and change the face of medicine. 高友鹤 YOUHE GAO Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. Advantages of Urine Proteome. Non-invasive Simpler than plasma Reflects changes of plasma Close to kidney and prostate.

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URIMEM, will facilitate biomarker discovery and change the face of medicine

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  1. URIMEM, will facilitate biomarker discovery and change the face of medicine 高友鹤 YOUHE GAO Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences

  2. Advantages of Urine Proteome • Non-invasive • Simpler than plasma • Reflects changes of plasma • Close to kidney and prostate

  3. Urine Proteome Studies

  4. Disadvantages • Big variation • Need more cases to validate

  5. Urine: may even better than blood for biomarker study • Urine • Suppose to be waste • Changes were maintained if not amplified • Blood • Suppose to be homeostatic • Changes were minimized

  6. Problems • More sample • Higher throughput MS • More analyzing power

  7. Problems to save samples • Very diluted • Protein degradation

  8. Possible ways to save • Precipitate: organic solvent consuming • Freeze dry: energy and labor consuming • Extraction: cost • Not suitable for large numbers of samples

  9. ( ( Urimem

  10. Urimem Urinary proteins dried on a filter and stored in vacuum

  11. Why is that important?

  12. Changed the face of medicine • “Hard as it is to believe today, a single concept developed by Dr. Henry Plummer  (1874 – 1936) at the beginning of the 20th century changed the face of medicine.” http://www.mayoclinic.org/tradition-heritage/medical-records.html

  13. Changed the face of medicine • The concept was a centralized medical record, stored in a single repository, and capable of traveling with the patient. (1907) • Now • paperless • filmless • chartless http://www.mayoclinic.org/tradition-heritage/medical-records.html

  14. Biobanking: foundation of personalized medicine • Blood • Urine • Skin cells • Organ • Tissue • other things taken from a body Hewitt RE. CurrOpinOncol. 2011 Jan;23(1):112-9. doi: 10.1097/CCO.0b013e32834161b8.

  15. Do we have enough? Why not? • Invasiveness • Storage expense • Limited biological information (hair, nail)

  16. URIMEM is the answer • Invasiveness: You can’t hold your urine for a few hours. • Storage expense: Proteins dried on a membrane can be stored in vacuum even at room temperature for quite a while. • Limited biological information: much more complicated information from the whole body especially from the secretory system

  17. Can we decode urine proteome? • Not yet • But having large number of samples will help

  18. Acknowledgement • Lulu Jia • Xuejiao Liu • Liu Liu • YouheGao高友鹤 • gaoyouhe@pumc.edu.cn • 13910861559 • Department of Nephrology, PUMC Hospital: Mingxi Li • Funding from 973 Project

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