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Proteomics of Neuroregeneration: Discovery of a Biomarker of Brain Repair

Proteomics of Neuroregeneration: Discovery of a Biomarker of Brain Repair.

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Proteomics of Neuroregeneration: Discovery of a Biomarker of Brain Repair

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  1. Proteomics of Neuroregeneration:Discovery of a Biomarker of Brain Repair Quantitative proteomics of mouse brain during post-traumatic regeneration2-D gel analysis of proteins in regenerating brain. The enlarged boxes in the 2-D gels show three spot intensities that were significantly increased in the injured hippocampus. High-resolution proteomic data showed strong upregulation of the protein 14-3-3 during healing.We conclude that the isotope-based quantitative proteomic method is a highly useful approach to assess protein expression levels in defined parts of the brain after specific brain injury. AcknowledgementsNSF DMR 0084173, Knut and Alice Wallenberg Foundation (C.L. Nilsson, P.I.) 14-3-3 quantification by use of an isotopic approach. Average peptide ratios were compared between contralateral and injured hippocampus of wildtype (WT) and transgenic(GV)mice. A 2D gel study of mouse hippocampus (a brain region): Identity of the three increased protein isoforms: 14-3-3 Sihlbom, C.; Wilhelmsson, U.; Li, L.; Nilsson C.L. and Pekny, M. « 14-3-3 Expression in Denervated Hippocampus after Entorhinal Cortex Lesion Assessed by Culture-Derived Isotope Tags in Quantitative Proteomics» J. Proteome Res. (2007), 6, 3491-3500.

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