Identification of Transcription Factor Binding Sites. Lior Harpaz Ofer Shany 09/05/2004. Goal - find TFBS !. input. output. Importance. TF regulate gene expression. Identification of TF can teach us: Mapping of regulatory pathways Potential functions of genes. Experimental Methods.
Identification of Transcription Factor Binding Sites
Conservation = functional importance
Total motifs: 80,000
P-value filter: 12,000
Low-complexity filter: 7,673
Hierarchically clustering: 1,269
34/48 known sites discovered.
Large fraction of matches for significant p-values.
Identification of known binding sites