In vitro morphogenetic responses of fenugreek and basil explants
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In Vitro Morphogenetic Responses of Fenugreek and Basil Explants. By: Jelisa Thomas Food and Animal Science Mentor: Dr. Leopold Nyochembeng. Background Information. Interesting Facts. FENUGREEK is an herb known for its high medicinal value. it originated from India

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In vitro morphogenetic responses of fenugreek and basil explants

In Vitro Morphogenetic Responses of Fenugreek and Basil Explants

By: Jelisa Thomas

Food and Animal Science

Mentor: Dr. Leopold Nyochembeng



Interesting facts
Interesting Facts Explants

FENUGREEK

  • is an herb known for its high medicinal value.

  • it originated from India

  • grown in Europe and Asia

    Basil

  • This herb is native to India and Persia

  • approximately 150 different species.


Fenugreek uses
Fenugreek uses Explants

  • Medically

    • Soar Throat

  • Culinary

    • Component in Curry etc.

  • Therapeutically

    • Oils

    • Soaps, etc


Basil uses
Basil Uses Explants

  • Culinary

    • flavor used in various cuisines

  • Medically

    • internally and externally

  • Therapeutically

    • oil or soaps


Objectives
objectives Explants

To develop a protocol for callus production and cell culture on Fenugreek explants

Assess effects of genotype and media on basil plantlet multiplication



Media explants preparation and culture
Media, Explants Preparation Explants, and Culture


Seed germination
Seed Germination Explants

Germination medium contained

half strength M&S (Murashige and Skoog 1962) salt,

7.5 g/L sucrose

4 g/L agar

pH. 5.5-6.0

Autoclaving at 15psi and 121°C for 20 min.

Disinfestations

30% Clorox bleach (1.8% NaOCl) containing 1 drop of Tween 20 under vacuum [600-700mmHg] for 20 minutes.

Rinsed 3 changes of sterile distilled deionize water.

Blotted dry with sterile filter paper and place germination media

All cultures were sealed with parafilm and placed in an incubator at 23± 1°C and 16 hr/8 hr light/dark photoperiod.


Fenugreek explants
Fenugreek Explants Explants

Media

M&S salts

30 g/L sucrose

A factorial combination of

  • NAA (0.0, 1.0 mg/L)

  • Kinetin (0.0, 0.3, 1.0, 3.0 mg/L)

    Agar(8 g/L)

    pH 5.6-6.0

    autoclaving at 15psi and 121°C for 20 min.


Basil seedlings of 5 genotypes 23 82 62 74 and 83
Basil seedlings of 5 genotypes (23, 82, 62, 74, and 83) Explants

Media

M & S salts

30 g/L sucrose

a combination of

  • Kinetin (0.1, 1.0, 3.0 mg/L)

  • NAA (0.1 mg/L)

    Agar(8 g/L).

    pH 5.6-6.0

    Autoclaving at 15psi and 121°C for 20 min.

    The seedlings of each basil genotype were evenly distributed in all media.


All cultures were sealed with parafilm and placed in an incubator at 23± 1°C and 16 hr/8 hr light/dark photoperiod.


Data and analyses

All cultures were observed weekly for contamination, explants growth and differentiation.

The parameter that were measured included the number of roots formed, shoots, callus production, plantlet production

The data was analyzed by taking the averages of counts.  

Data and Analyses


Results and discussion
Results and Discussion explants growth and differentiation.


Conclusion

Such responses will help in determining the most suitable protocol for micropropagating these important medicinal plants

Choice of explants and the type of genotype used are important criteria for successful application of micropropagation and cell culture techniques in Fenugreek and basil respectively.

CONCLUSION


References

References protocol for

Jan Schooley- Ginseng and Medicinal Herbs Specialist/OMAF, “Basil” 2003 http://www.omafra.gov.on.ca/english/crops/ort/herbs/basil.htm accessed 14 July, 2009

James E. Simon, Purdue University, West Lafayette IN 47907-1165, USA, “Basil” 1995 http://www.hort.purdue.edu/newcrop/CropFactSheet/basil.html accessed 15 July, 2009

Jeanine M. Davis, Extension Horticultural Specialist Department of Horticultural ScienceNorth Carolina Cooperative Extension Service, North Carolina State University “Basil” 1997 http://www.ces.ncsu.edu/depts/hort/hil/hil-125.html access 14 July, 2009

S. Elliot “The Facts About Fenugreek Growing and Using Fenugreek” 2009 

http://herb-gardens.suite101.com/article.cfm/the_facts_about_fenugreek#ixzz0LrzADCmg accessed 14 July, 2009

AcramTaji, Prakrash P. Kumar, PrakashLakshmanan “Invitro plant Breeding” 2002 http://book.google.com/books?id=CQxsDZXHVuYC&dq=stages+of+microprogation&source=gbs_navlinks_s accessed 15 July, 2009

References


Acknowledgements
Acknowledgements protocol for

Dr. Leopold Nyochembeng

Dr. Elisa Moss

REU Supporters and Participators

Department of Plant and Soil Sciences

Department of Food an Animal Sciences


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