'Sample buffer' presentation slideshows

Reversible Phosphorylation of Proteins

Reversible Phosphorylation of Proteins. OH. Protein/Enzyme. Protein phosphatase. Protein kinase. Protein/Enzyme. PO 4. (Ser, Thr or Tyr). Cellular Processes:. Metabolism, contractility, membrane transport and secretion, transcription and translation of genes, cell division,

574 views • 26 slides

Overview of 2DE

Overview of 2DE. Complex mixture of proteins. Denature and solubilize in solution (Sample prep). Separate proteins by charge in first dimension (IEF). Separate proteins by size in second dimension (SDS-PAGE). Individual proteins isolated as distinct protein features within a gel matrix.

386 views • 19 slides

Overview of 2DE

Overview of 2DE. Complex mixture of proteins. Denature and solubilize in solution (Sample prep). Separate proteins by charge in first dimension (IEF). Separate proteins by size in second dimension (SDS-PAGE). Individual proteins isolated as distinct protein features within a gel matrix.

197 views • 19 slides

2-Dimensional Electrophoresis

2-Dimensional Electrophoresis. ????? ? ?. Proteomics Pathway. Proteomics Pathway. History of 2-DE. 1956 -paper & starch gel 1975 -Coupling of IEF & SDS-PAGE. IEF. +. SDS-PAGE. Sample preparation. Solubility, size, charge, isoelectric point

881 views • 16 slides

SDS-Page Part II

Electrophoresis and Visualization. SDS-Page Part II. Boiling Lysates. Proteins are being denatured by the combination of SDS and heat. Resultant proteins take on a rod-like shape and a uniform negative charge-to-mass ratio proportional to their molecular weight. Electrophoresis.

349 views • 15 slides

Comparative Proteomics Kit I: Protein Profiler Module

228 views • 14 slides

CROSSLINKING OF PROTEINS

254 views • 4 slides

Cloning a DNA segment from bacteriophage

Cloning a DNA segment from bacteriophage. Recombinant plasmid DNA purified from bacterial cells Started restriction digests last week Why digesting with EcoRI again? Why digesting with EcoRI + BamHI?. Cloning a DNA segment from bacteriophage.

150 views • 3 slides