2 dimensional electrophoresis
Download
Skip this Video
Download Presentation
2-Dimensional Electrophoresis

Loading in 2 Seconds...

play fullscreen
1 / 16

2-Dimensional Electrophoresis - PowerPoint PPT Presentation


  • 116 Views
  • Uploaded on

2-Dimensional Electrophoresis. 생명과학부 김 원. Proteomics Pathway. Proteomics Pathway. History of 2-DE. 1956 -paper & starch gel 1975 -Coupling of IEF & SDS-PAGE. IEF. +. SDS-PAGE. Sample preparation. Solubility, size, charge, isoelectric point

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about '2-Dimensional Electrophoresis' - angie


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
2 dimensional electrophoresis

2-Dimensional Electrophoresis

생명과학부

김 원

history of 2 de
History of 2-DE
  • 1956

-paper & starch gel

  • 1975

-Coupling of IEF & SDS-PAGE

slide5

IEF

+

SDS-PAGE

sample preparation
Sample preparation
  • Solubility, size, charge, isoelectric point
  • No universal method due to the diverse nature of samples.
  • Essential to minimize protein modifications.
  • Protease
  • Salt
tissue sample
Tissue sample
  • Break up the tissue while it is still frozen or at liquid nitrogen.
  • Pestle, Mortar, Homogenizer
  • In cells, washed in phosphate-buffered saline (PBS) and solubilized in sample buffer
  • Ultracentrifugation-DNA, RNA 제거
2 dimensional electrophoresis1
2-Dimensional Electrophoresis

1D (IEF)

Equilibration

Identification

2D (SDS-PAGE)

ad