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Absorption Spectroscopy

Absorption Spectroscopy. Topic 4 part 1 Biophysics. Energy and Absorption. Absorption is proportional to Get resonance at hf = ħ w = E b – E a. www.andor.com/chemistry/?app=189. Transition Probability and transition dipole moment. m ba = transition dipole moment =. - like.

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Absorption Spectroscopy

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  1. Absorption Spectroscopy Topic 4 part 1 Biophysics

  2. Energy and Absorption • Absorption is proportional to • Get resonance at hf = ħw = Eb – Ea www.andor.com/chemistry/?app=189

  3. Transition Probability and transition dipole moment mba = transition dipole moment = - like , it has three Cartesian coordinates

  4. Absorption Broadening • Every electronic state (eg S0 and S1) has several vibrational and rotational substates. • Each of the sharp peaks is further broadened by Doppler shifts andsolvent effects and quantum probabilities. pubs.rsc.org/ej/DT/2000/B002123G/

  5. I1 l I0 Reference Beam Splitter monochromator Detector I2 l Light Source I0 Sample with pathlength l Detector I l monochromator I0 Sample with pathlength l Detector Light Source Extinction Coefficient Or A(l) = log (I0/I) A(l) = Ce(l)l

  6. Cross Section and Connecting Macroscopic to Micropscopic • = sNA/1000

  7. Biopolymer absorption Protein Secondary Structure Protein Aromatics Blood http://life.nthu.edu.tw/~lslpc/biophys/hyperchorism.gif

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