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Yi-Ming Arthur Chen ( 陳宜民 ), M.D., Sc.D. Dean, Office of R & D

Molecular Epidemiology of HIV and HCV Infection in Taiwan and the Development of Most Sensitive Diagnostic Methods to Detect SARS Corona Virus and H1N1 Viruses. Yi-Ming Arthur Chen ( 陳宜民 ), M.D., Sc.D. Dean, Office of R & D Institute of Microbiology and Immunology

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Yi-Ming Arthur Chen ( 陳宜民 ), M.D., Sc.D. Dean, Office of R & D

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  1. Molecular Epidemiology of HIV and HCV Infection in Taiwan and the Development of Most Sensitive Diagnostic Methods to Detect SARS Corona Virus and H1N1 Viruses Yi-Ming Arthur Chen (陳宜民), M.D., Sc.D. Dean, Office of R & D Institute of Microbiology and Immunology AIDS Prevention and Research Center National Yang-Ming University

  2. Adults and children estimated to be living with HIV, 2008 Eastern Europe & Central Asia 1.5 million [1.4 – 1.7 million] Western & Central Europe 850 000 [710 000 – 970 000] North America 1.4 million [1.2 – 1.6 million] East Asia 850 000 [700 000 – 1.0 million] Middle East&North Africa 310 000 [250 000 – 380 000] Caribbean 240 000 [220 000 – 260 000] South & South-East Asia 3.8 million [3.4 – 4.3 million] Sub-Saharan Africa 22.4 million [20.8 – 24.1 million] Latin America 2.0 million [1.8 – 2.2 million] Oceania 59 000 [51 000 – 68 000] Total: 33.4 million (31.1 – 35.8 million)

  3. Figure. Classification of human immunodeficiency viruses (HIV). HIV-1 recombinants are categorized into two class: circulating recombinant forms (CRFs) and unique recombinant forms (URFs). Takebe et al, Pediatr Int 2004

  4. HIV-1 diversity in the worldwide epidemic.

  5. 聯合報2005年一月

  6. 台灣愛滋病毒感染者報告病例數依危險因子分析台灣愛滋病毒感染者報告病例數依危險因子分析 注射藥癮者癮者 異性戀者 男同志及男雙性戀者 Chen et al, Lancet, 369: 623-625, 2007.

  7. BC亞型基因重組病毒株（CRF07_BC）傳到台灣來的可能路徑BC亞型基因重組病毒株（CRF07_BC）傳到台灣來的可能路徑 Chen and Kuo, Lancet 2007.

  8. Molecular Epidemiology of HIV-1 Infection and Full-Length Genomic Analysis of Circulating Recombinant Form 07_BC Strains from Injection Drug Users in Taiwan Lin et al. JID 2007;195:1283-1293.

  9. Phylogenetic analysis of HIV-1 strains in Taiwanese IDUs. J Acquir Immune Defic Syndr 2010;53:425–439

  10. Sharing dissolved heroine solution is a neglected risk factor associated with HIV-1 infection J Acquir Immune Defic Syndr 2010;53:425–439

  11. Hepatitis C Virus • Genotypes 1~6 (30% variation), many subtypes (20% variation) HCV SEQUENCE DATA BASE

  12. Subjects • Selected all IDU samples which were infected with CRF07_BC before 2003 and random sampling CRF07_BC cases which was infected in 2004,2005 classified by northern, central, southern Taiwan each 50. • Selected all samples infected with HIV-1 B type and CRF01_AE CRF_07BC

  13. HCV genotyping using multiplex RT-PCR assay with specific primers J Formosa Med Asso, in press, 2010.

  14. Phylogenetic analyses of three HCV subtype 6w strains using maximum likelihood

  15. Diversity plot of the HCV RF_2b6w strain

  16. Summary of the Molecular Epi Studies • The Taiwanese CRF07_BC strains had 97% full-length sequence homology with the prototype from mainland China. CRF07_BC was first introduced into the southern region in 2002 and then spread to other regions in Taiwan in 2004. • With HIV-1 CRF07_BC disseminating in Taiwan IDUs, there were new HCV subtypes emerging in Taiwan, eg., 6w. • About 13% of HIV-1-infected IDUs had mixed HCV infection and multiplex RT-PCR with specific primers method is suitable for the detection of such mixed HCV infection.

  17. Localized Surface Plasmon Coupled Fluorescence Fiber-optic Biosensor and It’s Biomedical Applications (LSPCF-FOB) 表面電漿共振原理:藉由雷射激發金膜，而促使金膜產生表面電漿波，當coating在上面的antibody有binding上antigen protein時，其共振角會改變而detector就偵測反射角的變化來判斷是否有protein被抓住。

  18. Conventional antigen capture ELISA Successfully detected at concentrations as low as 12.5 - 25ng/ml in PBS. Successfully detected at concentrations as low as 0.78 – 1.56 ng/ml in diluted

  19. GST-N protein test in LSPCF fiber-optic biosensor Successfully detected at concentrations as low as 0.1pg/ml in PBS. Successfully detected at concentrations as low as 0.1pg/ml in diluted serum.

  20. National Yang-Ming University: Prof. Chen’s Lab: Jer-Yuan Shiu，Shwu-Jen Tzeng，Kuan-Hsuan Chen，Hsiao-Han Liu，Cheng-Ming Lee，Chia-Yen Chen ，Jung-Hsien Hung，Chia-Hung Yen ，Pei-Chun Chuang，Ying-Shiuan Li，Yi-Jen Liao，Ching-Ping Yang, Shih-Ping Liu,Shih-Yin Chen, Yu-Chuen Huang, Yu-Ching Lan Inst. of Env. and Occu. Health Sci.: Tsung-Yun Liu Department of Life Sci : Ting-Fen Tsai，Fen-Hwa Wong Inst. of Micro. and Immuno.: Chi-Hung Lin Inst. of Biophotonic: Fu-Jen Kao Inst. of Biochem. and Mol. Biol.: Yan-Hwa Wu Lee Academia Sinica: Ying-Hue Lee, Michael Hsiao, Chen Chang, Lee- Young Chau Chung-Hsing University: En-Pei Chiang Chang-Gung University: Ling-Ling Hsieh NHRI: Shiu-Feng Huang Taipei VGH:Anna Fen-Yau Li，Teh-Ying Chou, Wing-Wai Wong Taiwan CDC Jyh-Yuan Yang, Steve Kuo Acknowledgements Supported by NYMU, NSC, DOH and MOE

  21. Thank you for your attention and welcome to Taiwan

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