Regulation of lipid storage in saccharomyces cerevisiae
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Regulation of lipid storage in Saccharomyces cerevisiae. Shaelie Harper McDonough-Stukey Lab. A Little History . How do cells “know” when they have made enough lipid? Previously, Cheryl Jacobs had isolated a mutant (114) we believe is defective at sensing lipid amounts

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Regulation of lipid storage in saccharomyces cerevisiae

Regulation of lipid storage in Saccharomyces cerevisiae

Shaelie Harper McDonough-Stukey Lab


A little history
A Little History

  • How do cells “know” when they have made enough lipid?

  • Previously, Cheryl Jacobs had isolated a mutant (114) we believe is defective at sensing lipid amounts

  • My work this summer was to begin characterization of the mutant


Growth phenotype clumpy vs normal
Growth Phenotype(clumpy vs. normal)

  • Within a tetrad, it can vary what strains are clumpy and which aren’t

  • If the ratio is 2:2 within a tetrad, this means there is only 1 mutation

Clumpy

Normal


Gas chromatography data
Gas Chromatography data

  • To the top is a picture of the GC at Hope College

  • To the left is an example of the data collected by the GC

  • Each Peak represents a different fatty acid, and its relative amount present in each cell


Data tables
Data Tables

  • By creating data tables, it is easier to make comparisons and conclusions for each individual strain


Fatty acid amount and composition
Fatty acid Amount and Composition

  • These charts are compiled after analyzing the data from the GC and organizing it onto spreadsheets (shown on the previous slide), so we can compare different strains


Result summary
Result summary

  • Clumpy Growth is inherited in a single gene manner (2:2 inheritance)

  • Preliminary results indicate that elevated

  • fatty acid amount is correlated to growth

  • habit, and is a single gene mutation


Conclusions
Conclusions

  • By examining inheritance patterns we are able to see

    • Clumpy growth habit is correlated to elevated FA amount

    • Elevated FA amount is a recessive single gene trait


Credits
Credits

  • REACH

  • Dr. McDonough and Dr. Stukey

  • Rachel Van Kempen

  • Caitlin Rice


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