Regulation of lipid storage in saccharomyces cerevisiae
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Regulation of lipid storage in Saccharomyces cerevisiae. Shaelie Harper McDonough-Stukey Lab. A Little History. How do cells “know” when they have made enough lipid? Previously, Cheryl Jacobs had isolated a mutant (114) we believe is defective at sensing lipid amounts

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Regulation of lipid storage in Saccharomyces cerevisiae

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Regulation of lipid storage in Saccharomyces cerevisiae

Shaelie Harper McDonough-Stukey Lab


A Little History

  • How do cells “know” when they have made enough lipid?

  • Previously, Cheryl Jacobs had isolated a mutant (114) we believe is defective at sensing lipid amounts

  • My work this summer was to begin characterization of the mutant


Growth Phenotype(clumpy vs. normal)

  • Within a tetrad, it can vary what strains are clumpy and which aren’t

  • If the ratio is 2:2 within a tetrad, this means there is only 1 mutation

Clumpy

Normal


Gas Chromatography data

  • To the top is a picture of the GC at Hope College

  • To the left is an example of the data collected by the GC

  • Each Peak represents a different fatty acid, and its relative amount present in each cell


Data Tables

  • By creating data tables, it is easier to make comparisons and conclusions for each individual strain


Fatty acid Amount and Composition

  • These charts are compiled after analyzing the data from the GC and organizing it onto spreadsheets (shown on the previous slide), so we can compare different strains


Result summary

  • Clumpy Growth is inherited in a single gene manner (2:2 inheritance)

  • Preliminary results indicate that elevated

  • fatty acid amount is correlated to growth

  • habit, and is a single gene mutation


Conclusions

  • By examining inheritance patterns we are able to see

    • Clumpy growth habit is correlated to elevated FA amount

    • Elevated FA amount is a recessive single gene trait


Credits

  • REACH

  • Dr. McDonough and Dr. Stukey

  • Rachel Van Kempen

  • Caitlin Rice


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