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Molecular Cell Biology of the Yeast Saccharomyces cerevisiae

Molecular Cell Biology of the Yeast Saccharomyces cerevisiae. Lecture III: Molecular Logic of Asymmetry Zhang Yi, National Institute of Biological Sciences, 20080628. Cellular Anatomy. Mitosis V.S Meiosis. α. α. a. a. Mating Type Switch. Mating Type Specific Gene.

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Molecular Cell Biology of the Yeast Saccharomyces cerevisiae

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  1. Molecular Cell Biology of the Yeast Saccharomyces cerevisiae Lecture III: Molecular Logic of Asymmetry Zhang Yi, National Institute of Biological Sciences, 20080628

  2. Cellular Anatomy

  3. Mitosis V.S Meiosis α α a a

  4. Mating Type Switch

  5. Mating Type Specific Gene • MATa gene expression • a1 mRNA from MATa locus • Other a-specific gene expression is default • Driven by MCM1 homodimer • MAT gene expression • 1, 2 mRNA from MAT • 1 and 2 function • Repress a-specific genes • 2/MCM1 heterodimer • Stimulate -specific genes • 1/MCM1 heterodimer

  6. Mating Type Specific Gene Expression

  7. Mating Type Switch Mechanism: HO

  8. Mating Type Switch Mechanism: hetero-sequence switch

  9. Recombination Enhancer Sequence

  10. Sequence-Specific RE Effects

  11. RE Sequence Shows MatAlpha2 Binding Site

  12. RE Mutants Render Chromatin Structural Change wt … mt …

  13. A Mcm1p Binding Site in RE is Necessary for RE Function

  14. MatAlpha2 Interaction with Tup1/Ssn6 is Essential for Haploid/a-specific Gene Repression

  15. Tup1/Ssn6 Complex is Global Transcription Suppressor Associated with HDACs

  16. A Model Summarizing Mcm1p-Mediated Chromosomal Effects on RE and Other Loci

  17. Evolution of the Molecular Logic of Mating Type in Fungi S.Pombe

  18. Maternal Effect on Mating Type Switch ???

  19. HO Promoter Dissection

  20. HO Expression Depends on Swi/Snf Complex: LOF and GOF

  21. Ash1p Represses Mating Type Switch in Daughter Cell by Inhibiting HO Expression

  22. Ash1p and Swi/Snf Show Reciprocal Effects on Mating Type Switch

  23. Ash1p Localization Dependence: Genetic Screen Screen for mother cells who do NOT express HO (promoter is inhibited) as a consequence of any genetic defect

  24. She Genes are Necessary for HO Expression

  25. She1p/5p are Cytoskeletal Proteins, and Some She Genes are Localized to Daughter Cells Interdependence of She genes on localization

  26. Actin-dependent Ash1 mRNA Localization into Daughter Cell Pharmacology LOF FISH Genetics GOF Genetics LOF

  27. She genes and Ash1 mRNP transport

  28. Visualizing mRNA Transport

  29. Local Translation

  30. Local Translation Regulation is as Essential as Localization

  31. Protein Level in Temporal Context Degredation for old alpha2 as well as HO etc

  32. Protein Dynamics not Limited to Ash1 mRNA Transportation

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