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Work package 7 Establishing new product/processes for bioremediation

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Work package 7 Establishing new product/processes for bioremediation. Partners: UMIL (2 PM) IAMAC (11 PM) UGENT (26 PM) – WP Leader ETS (19 PM) UNIBO (34 PM). Objectives. Development of biotechnological processes based on microorganisms from the collections (WP4):

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Presentation Transcript
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Work package 7

Establishing new product/processes for bioremediation

Partners:

  • UMIL (2 PM)
  • IAMAC (11 PM)
  • UGENT (26 PM) – WP Leader
  • ETS (19 PM)
  • UNIBO (34 PM)
objectives
Objectives

Development of biotechnological processes based on microorganisms from the collections (WP4):

  • the biosynthesis of valuable fine chemicals and new biosurfactantsby hydrocarbon degrading microbe
  • the reductive dehalogenation of chlorinated compounds with the new isolates
  • the reductive dehalogenation of PCBs by biopalladium
  • biotransformation of metals and metalloids in polluted sediments and definition of models describing their fate
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Task 7.1 - hydrocarbon degradation enzymes and microorganisms to obtain valuable chemicals – focus on biosurfactant
  • isolates and mixed microbial cultures obtained in WP4
  • enzymes obtained by the metagenomic approach of WP6
  • screened by using standard substrates and micro-array mapping
  • testing assays will involve both 96-well colorimetric, and high-throughput HPLC, GC and Maldi TOF MS/MS
  • 1L stirred tank bioreactor under batch conditions (10 strains)
  • Ecotoxicity of the biosurfactants
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Task 7.2 - Development of small scale bioprocesses for the reductive dehalogenation of chlorinated compounds
  • Develop biofilters for seawater contaminated with Cl-solvents
    • Chemical measurements
    • Ecotoxicityof the biosurfactants
  • Slurry bioreactors for the ex-situ removal of PCB
    • Biotransformation of PCBs
  • Testing assays will involve both 96-well colorimetric, and high-throughput HPLC, GC and Maldi TOF MS/MS
task 7 3 development of novel biopalladium nanoparticles dechlorination processes
Task 7.3 - Development of novel biopalladium nanoparticles dechlorination processes
  • Optimize the production of bio-Pd using different isolates
    • Size & properties of the nanocatalysts
  • Optimize the biogenic hydrogen production
  • Larger scale reactor systems
  • - Cl-solvents analysis
  • - PCB analysis
task 7 4 definition of a fate model for metal and metalloids in polluted sediments
Task 7.4 - Definition of a fate model for metal and metalloids in polluted sediments
  • Modeling of the fate of metals and metalloids
  • Based on sediments previously sampled and characterized in WP2 and WP3
    • Biotreatmentexperiments
    • Data elaboration and ecological modelling of the bioremediation process
time table wp7
Time table WP7

Start month: 12;End month: 35

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