Midterm policies
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Midterm Policies. Location: No Calculators Bring IDs Restroom Policy: Bring exam up to TA 1person gone at a time Come up to TA to ask a question. Office Hour Changes: in 4077A Young Hall. This week ONLY Canceled: Fri 10-11am Additional: Thurs 4-6pm. Weak Acids.

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Midterm Policies

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Midterm policies

Midterm Policies

  • Location:

  • No Calculators

  • Bring IDs

  • Restroom Policy:

    • Bring exam up to TA

    • 1person gone at a time

  • Come up to TA to ask a question


Office hour changes in 4077a young hall

Office Hour Changes:in 4077A Young Hall

  • This week ONLY

  • Canceled: Fri 10-11am

  • Additional: Thurs 4-6pm


Weak acids

Weak Acids

The weak acid H3PO4has 3 ionizablehydrogens, with the pKas: 2.15, 6.82, 12.38

draw the titration curve for: H+ vs pH

pOHvs H+

you make a solution by adding 0.5moles of HPO42- and 0.1 moles of PO43- to 1 Liter of water

What is the pH of the solution? Is it a good buffer?

You add 0.3 moles of NaOH.

What is the new pH of the solution?

Is it still a good buffer?


Amino acids peptides

Amino acids/Peptides

  • pKas: COOH=2, Lys = 10.5, Glu = 4.07, NH3=9.5

  • Draw the following peptide at physiological pH: Lakes

    • What is the net charge of the peptide?

    • What is the pI of the peptide?

  • Draw the titration curve for Glutamate (pH vs OH)


Sequencing what are these compounds and what do they do

Sequencing:What are these compounds and what do they do?

LiBH4


Sequencing

Sequencing

  • Chymotrypsin:

    • 1: Ile-Ser

    • 2: Leu-Lys-Val-Phe

    • 3: Ala-Arg-Gly-Try

  • Trypsin:

    • 1: Gly-Trp-Leu-Lys

    • 2: Val-Phe-Ile-Ser

    • 3: Ala-Arg


Purification

Purification

  • What are the 5 characteristics that we can use to separate proteins from each other?

  • What process can be used for each characteristic?


Protein structure

Protein structure

  • What are the levels of protein structure?

    • For each:

      • What interactions stabilize them?

      • How can we determine the structure?

    • Diagram Alpha helices and beta sheets

      • How do you get amphipathic structures in each?


Protein regulation

Protein regulation

  • What is cooperativity?

  • What is allosteric regulation?

    • What are the regulators of Hb? And what type of regulators are they?


Myoglobin hemoglobin

Myoglobin/Hemoglobin

  • How do Mb and Hb differ in how they bind oxygen?

  • How does this relate to their different biological functions?

  • How does destabilizing the T-state affect oxygen binding to Hb?

  • How does destabilizing the R-state affect oxygen binding to Hb?


Carbohydrates

Carbohydrates

  • What is the structure of glycogen?

    • What is its biological function and how does its structure relate to this?

  • How is the structure of Cellulose different from Glycogen?

    • How is this better for its biological function?


Lipids

Lipids

  • How are triacylglycerols better suited for long term energy storage than glycogen?

  • A fish has traveled to colder water. How would it change the composition of its membranes?


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