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Zestern analysis

Zestern analysis. A new method to address cross-reactivity of antibodies. Cross-reactivity of antibodies. One antibody, one protein (antigen) is the basis of immunoblot analysis. One antibody, two or more proteins ( antigen s ) is the reality.

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Zestern analysis

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  1. Zestern analysis A new method to address cross-reactivity of antibodies

  2. Cross-reactivity of antibodies One antibody, one protein (antigen) is the basis of immunoblot analysis One antibody, two or more proteins (antigens) is the reality. Majority of antibodies for research purpose actually recognize more than one protein (Cross-reactivity)*. *531 out of 22,000 antibodies are found without cross-reactivity Schwenk et al. Mol. & Cell Proteomics 9:2497-2507,2010. cross-reactivity of antibodies reflectedin Western blot analysis Antibody w/o Cross-reactivity Antibody w/ Cross-reactivity

  3. Traditional methods to address cross-reactivity of antibodies • Western blot analysis: Gel electrophoresis • Dot blot analysis • ELISA • Protein microarrays The number of samples can be analyzed are limited due to gel electrophoresis step Extensive screening to identify antibodies without cross-reactivity • The number of suitable antibodies are limited. • Insufficient coverage • high developmental cost

  4. Introducing Zestern analysis

  5. Basic of Zestern analysis Excessive antigen Excessive partial antigen Synthesized epitope peptide Synthesized epitope repeat Peptide Antibody Antigen Nitrocellulose membrane Epitope

  6. How Zestern analysis achieves specificity

  7. Advantages over traditional immunoblot analysis • Eliminates gel electrophoresis step in Western Blot analysis. • Increases number of antibodies available for Dot blot, ELISA and protein microarray analysis. • Increases the coverage of proteins for Dot blot, ELISA and protein microarray analyses. • Serves as foundation for high throughput protein analysis. • Provides basis for automation of immunoblot analysis.

  8. Further Info. • Zestern Biotechnique LLC owns US patent for Zestern analysis. • PCT application has been filed by Zestern Biotechnique LLC. • For further inquiry, contact: • Dr. Jiandi Zhang • Zesternbiotech@gmail.com • (001)-703-425-6666.

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