Determination of ubiquitin dimerization kinetics via UV spectrophotometry. Joel Prince Advisor: Ronald T. Raines Dept. of Biochemistry, University of Wisconsin–Madison. Outline. Background Ubiquitin Ubiquitin code and signals Research goal Experimental Approach
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Determination of ubiquitin dimerization kinetics via UV spectrophotometry
Advisor: Ronald T. Raines
Dept. of Biochemistry, University of Wisconsin–Madison
with respective % linkages in vivo
Xu, P. et. al. J. Cell.2009. 137, 133–145.
F. Ikeda, I. Dikic. EMBO Reports. 2008. 9, 536–542
Ub76 K C variant
NTB (412 nm)