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1. Inhibition of protein synthesis PowerPoint PPT Presentation


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+ Actinomycin D (5 m g/ml). 30’. 60’. 120’. 0’. Mamo et al.,. Supplementary Figure 1. Test sample. D t = 8 hours. 1. Inhibition of protein synthesis. 2. Inhibition of De novo transcription. + Emetine (100 m g/ml). Breast cancer cell lines. + Actinomycin D (5 m g/ml).

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1. Inhibition of protein synthesis

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1 inhibition of protein synthesis

+ Actinomycin D (5mg/ml)

30’

60’

120’

0’

Mamo et al.,

Supplementary Figure 1

Test sample

Dt = 8 hours

1. Inhibition of

protein synthesis

2. Inhibition of

De novo transcription

+ Emetine

(100mg/ml)

Breast cancer

cell lines

+ Actinomycin D (5mg/ml)

Dt = 8 hours

- Emetine

Control sample

0’

30’

60’

120’

Total RNAs extracted from

Test sample Control sample

Agilent cDNA

expression

microarray (44k)

Labelling

Cy3

Cy5


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