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+ Actinomycin D (5 m g/ml). 30’. 60’. 120’. 0’. Mamo et al.,. Supplementary Figure 1. Test sample. D t = 8 hours. 1. Inhibition of protein synthesis. 2. Inhibition of De novo transcription. + Emetine (100 m g/ml). Breast cancer cell lines. + Actinomycin D (5 m g/ml).
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+ Actinomycin D (5mg/ml) 30’ 60’ 120’ 0’ Mamo et al., Supplementary Figure 1 Test sample Dt = 8 hours 1. Inhibition of protein synthesis 2. Inhibition of De novo transcription + Emetine (100mg/ml) Breast cancer cell lines + Actinomycin D (5mg/ml) Dt = 8 hours - Emetine Control sample 0’ 30’ 60’ 120’ Total RNAs extracted from Test sample Control sample Agilent cDNA expression microarray (44k) Labelling Cy3 Cy5
